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12. Implications of QRS duration in dogs with pacing-induced heart failure
- Creator:
- Wang, Y., Gong, X., Su, Y., Cui, J., and Shu, X.
- Format:
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, echokardiografie, echocardiography, dyssynchrony, prognosis, remodeling, 14, and 612
- Language:
- English
- Description:
- The objective of this study was to find out the implication of QRS duration in dogs with rapid pacing-induced heart failure. Sixteen Beagle dogs were implanted with transvenous cardiac pacemakers and underwent rapid right ventricular pacing for 3 weeks at 260 bpm to induce hear t failure. Dogs were divided into two groups according to the QRS duration: 9 with normal QRS duration (<100 ms) and 7 wi th prolonged QRS duration (≥100 ms). Cardiac systolic functi on and size was analyzed by real time 3-dimensional echocardiography and left ventricular dyssynchrony was assessed by speckle tracking strain imaging. Congestive heart failure developed 3 weeks after rapid right ventricular pacing. Dogs with prolonged QRS duration showed more extensive radial strain and circumferential strain dyssynchrony than dogs with norm al QRS duration. At the end of 4-week recovery, greater improvemen t of left ventricular ejection fraction and left ventricular end-systolic volume was detected in dogs with normal QRS duration. The findings suggested that left ventricular dyssynchrony, indicated by a prolonged QRS duration, predicted an unsatisfying recovery in dogs with rapid pacing- induced heart failure. QRS duration had the potential to be a prognostic indicator for dogs with heart failure., Y. Wang ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
13. Improvement in the photoprotective capability benefits the productivity of a yellow-green wheat mutant in N-deficient conditions
- Creator:
- Zhang, X. H., Li, H. X., Zhuo, G., He, Z. Z., Zhang, C. Y., Shi, Z., Li, C. C., and Wang, Y.
- Format:
- počítač and online zdroj
- Type:
- model:article and TEXT
- Subject:
- nitrogen application rate, photoprotection, photosynthetic N-use efficiency, wheat, and yellow-green mutant
- Language:
- Multiple languages
- Description:
- Wheat yellow-green mutant Jimai5265yg has a more efficient photosynthetic system and higher productivity than its wild type under N-deficient conditions. To understand the relationship between photosynthetic properties and the grain yield, we conducted a field experiment under different N application levels. Compared to wild type, the Jimai5265yg flag leaves had higher mesophyll conductance, photosynthetic N-use efficiency, and photorespiration in the field without N application. Chlorophyll a fluorescence analysis showed that PSII was more sensitive to photoinhibition due to lower nonphotochemical quenching (NPQ) and higher nonregulated heat dissipation. In N-deficient condition, the PSI acceptor side of Jimai5265yg was less reduced. We proposed that the photoinhibited PSII protected PSI from over-reduction through downregulation of electron transport. PCA analysis also indicated that PSI photoprotection and electron transport regulation were closely associated with grain yield. Our results suggested that the photoprotection mechanism of PSI independent of NPQ was critical for crop productivity.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
14. KATP channels are involved in regulatory volume decrease in rat cardiac myocytes
- Creator:
- Leiyu Shi, Xu, M., Liu, J., Zhang, Z., Bao, Z., Wang, Y., Wang, C., and Wang, Jun
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, whole-cell patch clamp, ATP-sensitive K+ channel, regulatory volume decrease, 14, and 612
- Language:
- English
- Description:
- Regulatory volume decrease (RVD) is essential for the survival of animal cells. The aim of this study was to observe the RVD process in rat ventricular myocytes, and to determine if the KATP channels are involved in the RVD process in these cells. By using reverse transcriptase polymerase chain reaction and Western blot analysis, we demonstrated that there are two types of KATP channels expressed in rat ventricular myocytes: Kir6.1 and Kir6.2. When rat cardiac myocytes were exposed to hypotonic solution, cell volume increased significantly within 15 min and then gradually recovered. This typical RVD process could be inhibited by a Cl– channel blocker (0.5 mM 9-anthracene-carboxylic acid , 9-AC), a K+ channel blocker (5.0 mM CsCl) and a KATP channel blocker glibenclamide (10 μM). Electrophysiological results showed that hypotonic solution activated a whole-cell current, which had similar biophysical characteristics with KATP opener (pinacidil)-induced currents. This current could be blocked by glibenclamide. Our data suggested that the RVD process in rat ventricular myocytes is dependent on the activation of K+ channels, and that KATP channels are involved in this process., L. Shi ... [et al.]., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
15. Limonitum ameliorates castor oil-induced diarrhoea in mice by modulating gut microbiota
- Creator:
- Ma, Y. L., Yan, B. F., Liu, S. J., Dai, S. L., Liu, J., Wang, X. X., Fang, F., Wu, S. C., Wang, Y., Xu, C. Y., Zhao, Q., Wang, H. B., and Wu, D. K.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- traditional Chinese medicine, mineral Chinese medicine, mineral drug, mineral medicine, diarrhoea, Limonitum, gut microbiota, and short-chain fatty acids
- Language:
- English
- Description:
- Diarrhoea is a common clinical condition; its pathogenesis is strongly associated with gut microbiota dysbiosis. Limonitum is a well-known traditional Chinese medicine that exerts appreciable benefits regarding the amelioration of diarrhoea. However, the mechanism through which Limonitum ameliorates diarrhoea remains unclear. Here, the efficacy and underlying mechanism of Limonitum decoction (LD) regarding diarrhoea were explored from the aspect of gut microbiota. Castor oil (CO) was used to induce diarrhoea in mice, which were then used to evaluate the effects of LD regarding the timing of the first defecation, diarrhoea stool rate, degree of diarrhoea, diarrhoea score, intestinal propulsive rate, and weight of intestinal contents. The concentrations of short-chain fatty acids (SCFAs), including acetic, propionic, isobutyric, butyric and valeric acids, were analysed by gas chromatography-mass spectrometry (GC-MS). The 16S rRNA high-throughput sequencing technology was applied to evaluate changes in the gut microbiota under exposure to LD. LD was found to effectively ameliorate the symptoms of diarrhoea, and the diversity and relative abundance of gut microbiota were restored to normal levels following LD treatment. Additionally, LD significantly restored the observed reductions in SCFAs. These results provide strong evidence that LD can sufficiently ameliorate diarrhoea in mice by regulating their gut microbiota. The findings presented here highlight that Limonitum may constitute a prospective remedy for diarrhoea.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
16. Neuregulin-1 protects against doxorubicin-induced apoptosis in cardiomyocytes through an akt-dependent pathway
- Creator:
- An, T., Zhang, Y., Huang, Y., Zhang, R., Yin, S., Guo, X., Wang, Y., Zou, C., Wei, B., Lv, R., Zhou, Q., and Zhang, J.
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, neuregulin, doxorubicin, apoptosis, cardiomyocyte, akt, 14, and 612
- Language:
- English
- Description:
- In previous studies, it has been shown that recombinant human neuregulin-1(rhNRG-1) is capable of improving the survival rate in animal models of doxorubicin (DOX)-induced cardiomyopathy; however, the underlying mechanism of this phenomenon remains unknown. In this study, the role of rhNRG-1 in attenuating doxorubicin-induce apoptosis is confirmed. Neonatal rat ventricular myocytes (NRVMs) were subjected to various treatments, in order to both induce apoptosis and determine the effects of rhNRG-1 on the process. Activation of apoptosis was determined by observing increases in the protein levels of classic apoptosis markers (including cleaved caspase-3, cytochrome c, Bcl-2, BAX and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining). The activation of Akt was detected by means of western blot analysis. The study results showed that doxorubicin increased the number of TUNEL positive cells, as well as the protein levels of cleaved caspase-3 and cytochrome c, and reduced the ratio of Bcl-2/Bax. However, all of these effects were markedly antagonized by pretreament with rhNRG-1. It was then further demonstrated that the effects of rhNRG-1 could be blocked by the phosphoinositole-3-kinase inhibitor LY294002, indicating the involvement of the Akt process in mediating the process. RhNRG-1 is a potent inhibitor of doxorubicin-induced apoptosis, which acts through the PI3K-Akt pathway. RhNRG-1 is a novel therapeutic drug which may be effective in preventing further damage from occurring in DOX-induced damaged myocardium., T. An, ... [et al.]., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
17. Single leaves photosynthetic characteristics of two insect-resistant transgenic cotton (Gossypium hirsutum L.) varieties in response to light
- Creator:
- Sun, C. X., Qi, H., Hao, J. J., Miao, L., Wang, J., Wang, Y., Liu, M., and Chen, L. J.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- apparent light use efficiency, Bacillus thuringiensis, light-response curve, net photosynthetic rate, stomatal conductance, transgenic cotton, trypsin inhibitor, and water use efficiency
- Language:
- Multiple languages
- Description:
- How the photosynthetic characteristics of insect-resistant transgenic cotton (Gossypium hirsutum L.) respond to light or whether this genetic transformation could result in unintended effects on their photosynthetic and physiological processes is not well known. Two experiments were conducted to investigate the shapes of net photosynthetic rate (P N), stomatal conductance (g s), apparent light use efficiency (LUEapp) and water use efficiency (WUE) light-response curves for single leaves of Bt (Bacillus thuringiensis) and Bt+CpTI (cowpea trypsin inhibitor) transgenic cotton plants and their non-transgenic counterparts, respectively. Results showed that the significant difference in response of P N and WUE to light between transgenic cotton and non-transgenic cotton occured but not always throughout the growing season or in different experiments or for all transgenic cotton lines. It was highly dependent on growth stage, culture condition and variety, but no obvious difference between any transgenic cotton and non-transgenic cotton in the shapes of g s and LUEapp light-response curves was observed in two experiments at different growth stages. In the field experiments, transgenic Bt+CpTI cotton was less sensitive to response of P N to high irradiance at the boll-opening stage. In pot experiments, WUE light-response curves of both Bt transgenic cotton and Bt+CpTI transgenic cotton progressively decreased whereas non-transgenic cotton slowly reached a maximum at high irradiance at boll-opening stage. We supposed that culture environment could affect the photosynthesis of transgenic cotton both directly and indirectly through influencing either foreign genes expression or growth and physiological processes. and C. X. Sun ... [et al.].
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
18. Uniquely covered radical classes of $\ell$-groups
- Creator:
- Zhang, Y. and Wang, Y.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- radical class, atom, unique covering question, quasi-complement radical class, and $\sigma $-homogeneous
- Language:
- English
- Description:
- It is proved that a radical class $\sigma $ of lattice-ordered groups has exactly one cover if and only if it is an intersection of some $\sigma $-complement radical class and the big atom over $\sigma $.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
19. YBX1 protects against apoptosis induced by oxygen-glucose deprivation/reoxygenation in PC12 Cells via activation of the AKT/GSK3β pathway
- Creator:
- Tuerxun, T., Li, Xiaopeng, Hou, F., Wang, Y., Wang, X., and Ma, L.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- YBX1, AKT/GSK3β pathway, ischaemic stroke, cerebral ischaemia, and reperfusion injury
- Language:
- English
- Description:
- Reperfusion therapies for ischaemic stroke can induce secondary injury accompanied by neuronal death. The Y-box binding protein 1 (YBX1), an oncoprotein, is critical for regulating tumour cell proliferation and apoptosis. Thus, we wanted to know whether YBX1 could regulate neuronal cell apoptosis caused by cerebral ischaemia/reperfusion (I/R). We established a model of cerebral I/R-induced injury in vitro by oxygen-glucose deprivation/reoxygenation (OGD/R) treatment and determined YBX1 expression using Western blot. Next, the effect of YBX1 on the apoptosis and viability of OGD/R-treated PC12 cells was evaluated by flow cytometry, MTT assay, and Western blot. Besides, the release of lactate dehydrogenase (LDH) and the activity of catalase (CAT) and superoxide dismutase (SOD) were detected to evaluate oxidative stress of PC12 cells induced by OGD/R. The regulatory roles of YBX1 in the AKT/GSK3β pathway were examined by Western blot. As a result, OGD/R treatment down-regulated YBX1 expression in PC12 cells. YBX1 over-expression attenuated the growth inhibition and apoptosis of PC12 cells induced by OGD/R. Besides, the increase of LDH release and the decrease of SOD and CAT activities caused by OGD/R were reversed by YBX1 over-expression. Moreover, YBX1 over-expression could activate the AKT/GSK3β pathway in OGD/ R-treated PC12 cells. Therefore, YBX1 could protect against OGD/R-induced injury in PC12 cells through activating the AKT/GSK3β signalling pathway, and thus YBX1 has the potential to become a therapeutic target for cerebral I/R-induced injury.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public