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2. Neuregulin-1 protects against doxorubicin-induced apoptosis in cardiomyocytes through an akt-dependent pathway
- Creator:
- An, T., Zhang, Y., Huang, Y., Zhang, R., Yin, S., Guo, X., Wang, Y., Zou, C., Wei, B., Lv, R., Zhou, Q., and Zhang, J.
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, neuregulin, doxorubicin, apoptosis, cardiomyocyte, akt, 14, and 612
- Language:
- English
- Description:
- In previous studies, it has been shown that recombinant human neuregulin-1(rhNRG-1) is capable of improving the survival rate in animal models of doxorubicin (DOX)-induced cardiomyopathy; however, the underlying mechanism of this phenomenon remains unknown. In this study, the role of rhNRG-1 in attenuating doxorubicin-induce apoptosis is confirmed. Neonatal rat ventricular myocytes (NRVMs) were subjected to various treatments, in order to both induce apoptosis and determine the effects of rhNRG-1 on the process. Activation of apoptosis was determined by observing increases in the protein levels of classic apoptosis markers (including cleaved caspase-3, cytochrome c, Bcl-2, BAX and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining). The activation of Akt was detected by means of western blot analysis. The study results showed that doxorubicin increased the number of TUNEL positive cells, as well as the protein levels of cleaved caspase-3 and cytochrome c, and reduced the ratio of Bcl-2/Bax. However, all of these effects were markedly antagonized by pretreament with rhNRG-1. It was then further demonstrated that the effects of rhNRG-1 could be blocked by the phosphoinositole-3-kinase inhibitor LY294002, indicating the involvement of the Akt process in mediating the process. RhNRG-1 is a potent inhibitor of doxorubicin-induced apoptosis, which acts through the PI3K-Akt pathway. RhNRG-1 is a novel therapeutic drug which may be effective in preventing further damage from occurring in DOX-induced damaged myocardium., T. An, ... [et al.]., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3. Soil water content and photosynthetic capacity of spring wheat as affected by soil application of nitrogen-enriched biochar in a semiarid environment
- Creator:
- Yeboah, S., Zhang, R., Cai, L., Li, L., Xie, J., Luo, Z., Wu, J., and Antille, D. L.
- Format:
- print, bez média, and svazek
- Type:
- model:article and TEXT
- Subject:
- chemická hnojiva, produktivita plodin, zbytky plodin, výměna plynu, biochar, chemical fertilizers, crop productivity, crop residues, gas exchange, 2, and 581
- Language:
- Multiple languages
- Description:
- A field trial was conducted to determine the effect of nitrogen-enriched biochar on soil water content, plant’s photosynthetic parameters, and grain yield of spring wheat at the Dingxi Experimental Station during the 2014 and 2015 cropping seasons. Results showed that biochar applied with nitrogen fertilizer at a rate of 50 kg ha-1 of N (BN50) increased soil water content in the 0-30 cm depth range by approximately 40, 32, and 53% on average at anthesis, milking, and maturity, respectively, compared with zero-amendment (CN0). Stomatal conductance and net photosynthetic rate after the BN50 treatment increased by approximately 40 to 50% compared to CN0. Soil water content and photosynthetic traits also increased in other treatments using straw plus nitrogen fertilizer, but to lesser extent than that of BN50. Grain yields were highest (1905 and 2133 kg ha-1 in 2014 and 2015, respectively) under BN50. From this, biochar appears to have a potential for its use with N-fertilizer as a cost-effective amendment for crop production in semiarid environments., S. Yeboah, R. Zhang, L. Cai, L. Li, J. Xie, Z. Luo, J. Wu, D. L. Antille., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public