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1. Gastrodin ameliorates anxiety-like behaviors and inhibits IL-1β level and p38 MAPK phosphorylation of hippocampus in the rat model of posttraumatic stress disorder

Creator:
Peng, Z., Huan Wang, Zhang, R., Chen, Y., Xue, F., Nie, H., Wu, D., Wang, Y., Wang, H., and Tan, Q.
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, úzkost, stres (fyziologie), PTSD, gastrodin, IL-6, IL-1β, iNOS, p38, 14, and 612
Language:
English
Description:
Z. Peng ... [et. al.]. and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. The role of adrenergic agonists on glycogenolysis in rat hepatocyte cultures and possible involvement of NO

Creator:
Hodis, J., Nikolína Kutinová-Canová, Petr Potměšil, Ludmila Kameníková, Eva Kmoníčková, Zdeněk Zídek, and Hassan Farghali
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Experimentální medicína, farmakologie, oxid dusnatý, adrenalin, pharmacology, nitric oxide, adrenaline, glykogen, glykogenolýza, epinefrin, glycogen, glycogenolysis, epinephrine, iNOS, cAMP, 14, and 616-092
Language:
English
Description:
Certain liver metabolic diseases point to the presence of disturbances in glycogen deposition. Epinephrine raises the cAMP level that activates protein kinase A leading to the activation of phosphorylase and glycogen breakdown. In the present report, we sought to investigate whether NO is produced during adrenoceptor agonist-induced glycogenolysis in rat hepatocytes in cultures. Isolated glycogen rich rat hepatocytes in cultures were used. NO production (NO2-) was assessed under the effect of adrenergic agonists and adrenergic agonist/antagonist pairs, dibutyryl cyclic AMP sodium-potassium salt (db-cAMP), NO synthase (NOS) inhibitors Nω-nitro-L-arginine methyl ester (L-NAME), aminoguanidine (AG) and the NO donor S-nitroso-N-acetyl penicillamine (SNAP) . The inducible NO synthase (iNOS) mRNA was examined by the reverse transcription-polymerase chain reaction (RT-PCR). Glycogenolysis was quantified by glucose levels released into medium. The amount of glucose and NO2- released by hepatocytes was increased as a result of epinephrine, phenylephrine or db-cAMP treatments. The increase in glucose and NO2- released by epinephrine or phenylephrine was blocked or reduced by prazosin pretreatment and by NOS inhibitors aminoguanidine and L-NAME. iNOS gene expression was up-regulated by epinephrine. It can be concluded that glycogenolysis occurs through α adrenoceptor stimulation and a signaling cascade may involve NO production., J. Hodis, N. Kutinová-Canová, P. Potměšil, L. Kameníková, E. Kmoníčková, Z. Zídek, H. Farghali., and Obsahuje biblografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public