Chloroplasts are commonly the site of the earliest abiotic injury visible in plant ultrastructure. In this study, six inbred lines of maize (Zea mays L.) were used to analyze changes in the ultrastructure of chloroplasts and related physiological parameters under conditions of drought stress simulated by 20% polyethylene glycol 6000 (-0.6 MPa) for two days. Chloroplasts of three maize lines proved to be more sensitive. They showed changes in the ultrastructure in response to drought, including damage of thylakoid membranes, an increase in the number and size of plastoglobuli, swelling of thylakoid membranes both stromal and granal, disorganization of the thylakoid membrane system, an obvious increase in the intrathylakoid space, and a decrease in the
length-to-width ratio and area of chloroplasts. In addition, the contents of malondialdehyde increased markedly in the sensitive lines. Contrary to the sensitive lines, stable structures and shapes of chloroplasts were observed in the drought-resistant lines; it could be considered as an advantage contributing to drought tolerance in the plants. In addition, the drought index of leaf fresh mass (LMDI) in the drought-sensitive lines was ≤ 0.5, which was also associated with a lower content of leaf chlorophyll. In contrast, drought tolerance coincided with lesser growth reduction, and higher LMDI and leaf chlorophyll content., R. X. Shao, L. F. Xin, H. F. Zheng, L. L. Li, W. L. Ran, J. Mao, Q. H. Yang., and Obsahuje seznam literatury
We compared the effects of salt-stresses (SS, 1: 1 molar ratio of NaCl to Na2SO4) and alkali-stresses (AS, 1: 1 molar ratio of NaHCO3 to Na2CO3) on the growth, photosynthesis, solute accumulation, and ion balance of barley seedlings, to elucidate the mechanism of AS (high-pH) damage to plants and the physiological adaptive mechanism of plants to AS. The effects of SS on the water content, root system activity, membrane permeability, and the content of photosynthetic pigments were much less than those of AS. However, AS damaged root function, photosynthetic pigments, and the membrane system, led to the severe reductions in water content, root system activity, content of photosynthetic pigments, and net photosynthetic rate, and a sharp increase in electrolyte leakage rate. Moreover, with salinity higher than 60 mM, Na+ content increased slowly under SS and sharply under AS. This indicates that high-pH caused by AS might interfere with control of Na+ uptake in roots and increase intracellular Na+ to a toxic level, which may be the main cause of some damage emerging under higher AS. Under SS, barley accumulated organic acids, Cl-, SO4 2-, and NO3 - to balance the massive influx of cations, the contribution of inorganic ions to ion balance was greater than that of organic acids. However, AS might inhibit absorptions of NO3 - and Cl-, enhance organic acid synthesis, and SO4 2- absorption to maintain intracellular ion balance and stable pH. and C.-W. Wang ... [et al.].
Seedlings of Chloris virgata were treated with varying (0-160 mM) salt-stress (SS; 1 : 1 molar ratio of NaCl to Na2SO4) or alkali-stress (AS; 1 : 1 molar ratio of NaHCO3 to Na2CO3). To compare these effects, relative growth rates (RGR), stored energy, photosynthetic pigment contents, net photosynthetic rates, stomatal conductance, and transpiration rates were determined. Both stresses did not change significantly the photosynthetic parameters of C. virgata under moderate stress (below 120 mM). Photosynthetic ability decreased significantly only at high stress (160 mM). Thus C. virgata, a natural alkali-resistant halophyte, adapts better to both kinds of stress. The inhibition effects of AS on RGR and energy storage of C. virgata were significantly greater than that of SS of the same intensity. The energy consumption of C. virgata was considerably greater while resisting AS than while resisting SS. and C. W. Yang ... [et al.]
In the study of Tomlain (1997) a soil water balance model was applied to evaluate the climate change impacts on the soil water storage in the Hurbanovo locality (Southwestern Slovakia), using the climate change scenarios of Slovakia for the years 2010, 2030, and 2075 by the global circulation models CCCM, GISS and GFD3. These calculations did not take into consideration neither the various soil properties, nor the groundwater table influence on soil water content. In this study, their calculated data were compared with those monitored at the same sites. There were found significant differences between resulting soil water storage of the upper 100 cm soil layer, most probably due to cappilary rise from groundwater at sites 2 and 3. It was shown, that the soil properties and groundwater table depth are importat features strongly influencing soil water content of the upper soil layer; thus the application of the soil water balance equation (Eq. (1)), neglecting the above mentioned factors, could lead to the results far from reality. and V práci Tomlaina (1997) bol aplikovaný bilančný model vodného režimu pôd na ohodnotenie dopadu klimatickej zmeny na vodné zásoby pôdy v lokalite Hurbanovo (juhozápadné Slovensko), použijúc scenáre klimatickej zmeny pre Slovensko pre roky 2010, 2030 a 2075, založené na globálnych cirkulačných modeloch CCCM, GISS a GFD3. V týchto výpočtoch nebol braný do úvahy vplyv vlastností pôdy a hladiny podzemnej vody na vlhkosť pôdy. V práci boli porovnané vypočítané hodnoty zásob vody s monitorovanými v tej istej lokalite. Bol nájdený význačný rozdiel medzi zásobami vody v 100-cm hornej vrstve pôdy najpravdepodobnejšie spôsobený kapilárnym prítokom od hladiny podzemnej vody v monitorovacích miestach 2 a 3. Bolo ukázané, že pôdne vlastnosti a hĺbka hladiny podzemnej vody sú dôležitými faktormi, ktoré silno ovplyvňujú vlhkosť hornej vrstvy pôdy; z toho vyplýva, že aplikácia bilančnej rovnice (rov. (1)), ktorá zanedbáva vyššie uvedené faktory, nedáva reálne výsledky.
In Sedum wrightii grown in a growth chamber, detached leaves could survive for at least 120 d with a high rate of success for propagule formation. The pattern of gas exchange, associated with CAM, may be important in extending the period during which the detached leaf remains physiologically active. The added benefit for the developing propagule, still attached to the "parent" leaf, is an additional source of water and saccharide reserves over an extended period necessary for rooting. Drought survival of propagules may be determined by the amount of water-storing tissue in the detached leaf.
Soil salinization and alkalinization frequently co-occur in naturally saline and alkaline soils. To understand the characteristics of mixed salt-alkali stress and adaptive response of Medicago ruthenica seedlings to salt-alkali stress, water content of shoots, growth and photosynthetic characteristics of seedlings under 30 salt-alkaline combinations (salinity 24-120 mM and pH 7.03-10.32) with mixed salts (NaCl, Na2SO4, NaHCO3, and Na2CO3) were examined. The indices were significantly affected by both salinity and pH. The interactive effects between salt and alkali stresses were significant, except for photosynthetic pigments. Water content of shoots, relative growth rates of shoots and roots and pigment concentrations showed decreasing trends with increasing salinity and alkalinity. The root activity under high alkalinity and salinity treatments gradually decreased, but was stimulated by the combined effects of low alkalinity and salinity. The survival rate decreased with increased salinity, except at pH 7.03-7.26 when all plants survived. Net photosynthetic rate, stomatal conductance and intercellular CO2 concentration decreased with increased salinity and pH. M. ruthenica tolerated the stress of high salt concentration when alkali concentration was low, and the synergistic effects of high alkali and high salt concentrations lead to the death of some or all seedlings. M. ruthenica appeared to be salt-alkali tolerant. Reducing the salt concentration or pH based on the salt components in the soil may be helpful to abate damage from mixed salt-alkaline stress. and J. Y. Yang ... [et al.].
Non-destructive and rapid method for assessment of leaf photosynthetic characteristics is needed to support photosynthesis modelling and growth monitoring in crop plants. We determined the quantitative relationships between leaf photosynthetic characteristics and canopy spectral reflectance under different water supply and nitrogen application rates. The responses of reflectance at red radiation (wavelength 680 nm) to different water contents and nitrogen rates were parallel to those of leaf net photosynthetic rate (PN). The relationships of reflectance at 680 nm and ratio index of R(810,680) (near infrared/red, NIR/R) to PN of different leaf positions and leaf layers in rice indicated that the top two full leaves were the best leaf positions for quantitative monitoring of leaf PN with remote sensing technique, and the ratio index R(810,680) was the best ratio index for evaluating leaf photosynthetic characteristics in rice. Testing of the models with independent data sets indicated that R(810,680) could well estimate PN of top two leaves and canopy leaf photosynthetic potential in rice, with the root mean square error of 0.25, 0.16, and 4.38, respectively. Hence R(810,680) can be used to monitor leaf photosynthetic characteristics at different growth stages of rice under diverse growing conditions. and Y. Tian, Y. Zhu, W. Cao.
Hypergel is a novel material that uses a combination of several chemical structures which thus together create a relatively complex copolymer. It provides many improvements to contact lenses wearers related to comfort and biomechanical properties influence on the corneal tissue without the presence of silicone. This material uses knowledge coming from biomimetics. Thanks to its innovations this material is very positive evaluated by clients. and Hypergel je nový materiál využívající kombinaci několika chemických struktur, které dohromady tvoří relativně složitý kopolymer. Ten nabízí nositelům mnohá zlepšení týkající se komfortu při nošení kontaktních čoček a vlivu biomechanických vlastností na rohovkovou tkáň, a to bez přítomnosti silikonu. Tento materiál využívá poznatky z biomimetiky. Díky svým inovacím je klienty velmi kladně hodnocen.
In this study, the JIP test was used to assess the drought tolerance of two sweet cherry cultivars (Prunus avium L.) (modern and autochthonous). Plants were exposed to progressive drought by withholding water and their fast (< 1 s) chlorophyll fluorescence kinetics was evaluated. JIP test analysis showed that drought stress caused a greater decrease in performance indices (PIABS and PItotal) in a modern cultivar, as compared to an autochthonous one. Our results suggest that limited reoxidation of primary quinone electron acceptor (QA), higher amount of secondary quinone electron acceptor (QB-) nonreducing reaction centres, or inhibition of the electron transport between QA and QB, decreased more seriously the photosynthetic performance of the modern cultivar. Further, higher positive L- and K-bands observed for the modern cultivar also suggest lower energetic connectivity between PSII units and increased inhibition of oxygen-evolving complex over autochthonous cultivar. Our results suggest that the autochthonous cultivar Crveni hrušt had better photosynthetic performance under drought conditions, compared to the modern cultivar New Star.
The specific features of the structural and functional organisation of the photosynthetic apparatus (PSA) were studied in wild halophytes representing three strategies of salt tolerance: euhalophyte Salicornia perennans, crynohalophyte Limonium gmelinii, and glycohalophyte Artemisia santonica. The sodium content in aboveground parts of the plants corresponded to the strategy of salt tolerance. The photosynthetic cells of the euhalophyte were large and contained a higher number of chloroplasts than those in other species. In contrast, the number of cells per a leaf area unit was lower in S. perennans as compared to cryno- and glycohalophytes. Thereupon, the cell and chloroplast surface area per leaf area unit declined in the following sequence: A. santonica > L. gmelinii > S. perennans. However, the large cells of euhalophyte contained chloroplasts of larger sizes with 4- to 5-fold higher chlorophyll (Chl) content per chloroplast and Chl concentration in chloroplast volume unit. Also, chloroplasts of S. perennans were characterised by the higher content of glyco- and phospholipids. Qualitative composition of fatty acids (FA) in lipids isolated from the chloroplast-enriched fraction was similar in all three species; however, the index of unsaturation of FA was higher in glycohalophyte A. santonica than those in two other species. Under natural condition, PSA of all three halophytes showed high resistance to soil salinity. The results indicated tolerance of PSII to the photodamage in halophytes. The high rate of electron transport through PSII can be important to prevent oxidative damage of PSA in halophytes under strong light and hight temperature in vivo. Thus, the strategy of salt tolerance is provided by both the leaf anatomical structure and the ultrastructure of photosynthetic membranes, which is determined in particular by the specific composition of lipids., O. A. Rozentsvet, E. S. Bogdanova, L. A. Ivanova, L. A. Ivanov, G. N. Tabalenkova, I. G. Zakhozhiy, V. N. Nesterov., and Seznam literatury