The Czech Society for Histo- and Cytochemistry is a successor of The Czechoslovak Society for Histo- and Cytochemistry founded in 1966. The Society provides an interdisciplinary forum to support a study of the relationships among the structure, chemical composition and function in cells and tissues by histochemical, immunohistochemical and cytochemical methods. The Society encourages its members in the development and improvement of histochemical, immunohistochemical, and cytochemical methods used to discover the content and function of the tissue and cellular systems in situ and the application of the methods for diagnostics. and Jaroslav Mokrý.
As nitric oxide is considered a mediator of liver oxid ative metabolism during sepsis, we studied the effects of exogenous nitric oxide, produced by NO-donor, (±)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (NOR-3), on cell viability, urea biosynthesis and oxygen consumption in rat hepatocyte cultures. Nitric oxide release from NOR-3 was studied using 4,5-diaminofluorescein diacetate. Urea levels were measured by the spectrophotometric method. Cell viability was determined by the MTT test and trypan blue exclusion test, whereas oxygen consumption was measured by a polarographic technique. After 2 h treatment, NOR-3 induced an increase in the levels of nitric oxide. After 2 h of treatment and 24 h after the end of the treatment with NOR-3, both cell viability and urea synthesis were significantly reduced in comparison to the controls for NOR-3 concentrations equal to or greater than 50 μM. A reduction in oxygen consumption was observed in hepatocytes after 40 min treatment with 100 μM NOR-3, even if the cell viability was unchanged. Reduction of oxygen consumption is an early indicator of the metabolic alterations in hepatocytes exposed to nitric oxide. These findings suggest that nitric oxide accumulation acts on hepatocyte cultures inducing cell death and reduction of urea synthesis after 2 hours., R. Chimenti, G. Martino, S. Mazzulla, S. Sesti., and Obsahuje bibliografii a bibliografické odkazy
The influence of host intrapatch spatial distribution on parasitoid host acceptance behavior was investigated with Trichogramma principium parasitizing eggs of grain moth, Sitotroga cerealella. Single females were placed in Petri dishes, each containing 60 host eggs arranged either as a compact patch or partitioned into 60 or 12 clusters each consisting of 1 or 5 eggs, respectively. Partitioned patches provoked parasitization more often than compact patches. The percentage of ovipositing females (i.e., females parasitizing at least one of 60 host eggs) increased with the number of clusters, while it was independent of the intercluster distance over intervals of 2.5-15 mm. The mean number of eggs parasitized by ovipositing females during 48 h was almost independent of the host egg spatial pattern. As a result, the rate of parasitization was higher when the hosts were sparsely distributed within a patch than when they were aggregated., Nataliya D. Voinovich, Taisiya Ya. Umarova, Sergey Ya. Reznik, and Lit
Helminti, známí též jako červi, jsou v tradičním pojetí považováni za více či méně závažné parazity zvířat a člověka. Současný výzkum však postupně odhaluje i jejich druhou tvář a hledá jejich možné využití v moderní medicíně., Helminths, also known as worms, are traditionally considered to be more or less serious parasites for both animals and man. Nevertheless, current research has revealed some beneficial attributes and aims to uncover their potential application in modern medicine., and Tomáš Macháček, Jana Bulantová, Lucie Jedličková, Roman Leontovyč, Jan Pankrác, Vladimír Skála, Libuše Turjanicová, Petr Horák.
The apolipoprotein A-V (apo A-V) plays an important role in regulation of triglyceride (TG) concentration in serum. To better understand how apo A-V affects triglyceridemia and glucoregulation, the lipoprotein lipase (LPL) activity was determined using intravenous fat tolerance test (IVFTT) and oral glucose tolerance test (oGTT) was performed in carriers of apolipoprotein A-V gene ( APOAV) variants known to be associated with increased triglyceridemia. Twelve carriers of 19W variant, 16 carriers of -1131C variant, 1 combined heterozygote and 16 control subjects homozygous for wild type variants (19S/-1131T) were selected from a population sample and matched with respect to body mass index and age. The APOAV variants carriers had increased TG, very low density lipoprotein-TG, and apo B concentrations (p < 0.05). The LPL activity evaluated as k2 rate constant for clearance of Intralipid® was 14 % lower in APOAV variants carriers. The depression of nonesterified fatty acids (NEFA) concentration after glucose load was delayed in APOAV variants carriers in spite of the same insulinemia and glycemia. Our results suggest that variants of APOAV combined with increased triglyceridemia are associated with lower LPL activity in vivo and with disturbances of regulation of NEFA concentration after glucose load., J. Kovář, V. Adámková., and Obsahuje bibliografii a bibliografické odkazy
It is known that chromosomes occupy non-random positions in the cell nucleus. However, it is not clear to what extent their nuclear positions, together with their neighborhood, are conserved in daughter cells. To address specific aspects of this problem, we used the model of the chromosomes carrying ribosomal genes that are organized in clusters termed Nucleolus Organizer Regions (NORs). We compared the association of chosen NOR-bearing chromosomes (NOR-chromosomes) with nucleoli, as well as the numbers of nucleoli, in the pairs of daughter cells, and established how frequently the daughter cells had equal numbers of the homologs of certain NOR-chromosomes associated with individual nucleoli. The daughter cells typically had different numbers of nucleoli. At the same time, using immuno-FISH with probes for chromosomes 14 and 15 in HeLa cells, we found that the cell pairs with identical combinations appeared significantly more frequently than predicted by the random model. Thus, although the total number of chromosomes associated with nucleoli is variable, our data indicate that the position of the NOR-bearing chromosomes in relation to nucleoli is partly conserved through mitosis., M. Kalmárová, E. Smirnov, L. Kováčik, A. Popov, I. Raška., and Obsahuje bibliografii a bibliografické odkazy
V oblasti magnetického cielenia buniek, liečiv alebo génov, využívajúc princíp magnetickej separácie vel'kým gradientom, boli navrhnuté a teoreticky analyzované nové systémy, ako aj jednoduché usporiadania magnetov, už skôr študované Halbachovo pole. Na modelovanie magnetického pol'a sa s úspechom použiva metóda konečných prvkov. Táto metóda je predmetom tohto referátu. Novým potenciálnym systémom na magnetickú separáciu v mikrometrických rozemroch je nami študované pole s ve'lkým gradientom v okolí mikrometrických diskov zo silného feromagnetika v pôvodne homogénnom poli. Bol určený približný efektívny dosah takéhoto systému v prípade nanočastíc rádovo v mikrometroch. Obdobná analýza bola vykonaná aj pre magnetické částice v prúdiacom viskóznom tekutom prostredí. Účinná separácia, resp. cielenie, v prípade malých magnetov milimetrových rozmerov v priamom kontakte s kapilárou bola dosiahnutá pre mikročastice. V podobných systémoch v centimetrových rozemroch boli pozorované porovnatel'né výsledky. Kombinácia vel'kejj intenzity a gradientu je dosiahnutá len v blízkom okolí magnetov. Väčší dosah bol pozorovaný v prípade študovaného symetrického Halbachovho pol'a., The magnetic particles play an important role as magnetic drug, gene or cell carriers, in biomedicine and biotechnology. Their combination with a strongly non-homogeneous magnetic field is considered as a powerful way to target them to desired sites in living organism. It is important to know the potential applicability of these systems for magnetic drug targeting in living organism. For these purposes we have developed and evaluated a numerical model of magnetic targeting using finite element method applied to solve Maxwell equations describing electromagnetic field coupled with Newton law of particle motion., Andrej Krafčík, Melánia Babincová, Peter Babinec., and Obsahuje bibliografii