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1. Apoptotic damage of DNA in Hhuman leukaemic HL-60 cells treated with C2-ceramide was detected after G1 blockade of the cell cycle

Creator:
Bártová, E., Španová, A., Janáková, L., Bobková, M., and Rittich, B.
Type:
article, model:article, and TEXT
Subject:
HL-60 cells, C2-ceramide, apoptosis, and cell cycle
Language:
English
Description:
Apoptosis was induced by treatment of HL-60 cells with C2-ceramide. Apoptotic damage of DNA was detected according to the sub-Gl peak on a flow cytometer, according to the typical morphology and according to the DNA fragmentation "ladder" after gel electrophoresis. It was shown that the apoptotic cleavage followed after G1 blockade of the cell cycle. A high correlation coefficient (rs=0.957) was found between the percentages of G1 blocked cells and apoptotic cells. This high correlation together with the appearance of the sub-Gl peak suggests that the G1 blocked HL-60 cells were subject to apoptotic death. It is deduced that the mechanisms leading to G1 blockade of the cell cycle and activation of apoptosis in HL-60 cells are interconnected.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Expression of mRNA for adenosine A1, A2a, A2b, and A3 receptors in HL-60 cells: dependence on cell cycle phases

Creator:
Michal Hofer, Ladislav Dušek, Zuzana Hoferová, Lenka Stixová, and Milan Pospíšil
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, adenosinové receptory, buněčný cyklus, adenosine receptors, cell cycle, messenger RNA expression, HL-60 promyelocytic cells, 14, and 612
Language:
English
Description:
The present studies investigated changes in expression of mRNA for adenosine A1, A2a, A2b, and A3 receptors in samples of HL-60 promyelocytic cells differing in the actual presence of cells in various phases of the cell cycle induced by the double thymidine block method. Real-time PCR technique was used for obtaining data on mRNA expression. Statistical analysis of the data revealed that the mRNA ex pression of adenosine A1, A2a, and A3 receptors is dependent on the cell cycle phase. G0/G1 and G2/M phases were characterized by a higher mRNA expression of adenosine A1 receptors and a lower one of adenosine A2a and A3 receptors whereas the opposite was true for the S phase. Interestingly, expression of mRNA of the adenosine A2b receptors was independent on the cell cycle phase. The results indicate the plasticity of mRNA expression of adenosine receptors in the investigated promyelocytic cells and its interaction with physiological mechanisms of the cell cycle., M. Hofer ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Induction of cell-cycle inhibitor p21 in rat ventricular myocytes during early postnatal transition from hyperplasia to hypertrophy

Creator:
Horký, M., Kuchtíčková, Š., Vojtěšek, B., and Kolář, F.
Type:
article, model:article, and TEXT
Subject:
ventricular myocyte, cell cycle, proliferation, and postnatal development
Language:
English
Description:
To examine a possible involvement of p21 protein, an inhibitor of cyclin-dependent kinases (CDKs), in the transition from hyperplastic to hypertrophic growth of rat ventricular myocytes during the first postnatal week, we analysed day-by-day changes in the number of p21 positive cells using specific antibodies against this protein. Paraffin-embedded sections of the left ventricular myocardium were examined by means of immunoperoxidase technique and hematoxylin-eosin counterstaining. While during the first three postnatal days, the positive reaction for p21 was detected only in a small fraction of myocytes (12-20 %), a sudden increase in positivity occurred on day 4 (54 %) and continued till day 6 when the fraction of cells expressing p21 reached 87 %. Our results show that the induction of CDK inhibitor p21 in rat ventricular myocytes is developmentally regulated. Moreover, the fact that the sudden increase in p21 positivity occurred at the same stage when the myocyte proliferation rapidly ceases, suggests that this protein is likely to be involved in mediating this key event of cardiac development.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Neue Untersuchungen über die Reifung und Befruchtung

Creator:
František Vejdovský
Publisher:
Verlag der Königl. böhm. Gesellschaft der Wissenschaften
Format:
print, svazek, and 103 stran, ix listů obrazových příloh : ilustrace.
Type:
model:monograph and TEXT
Subject:
Buněčná biologie. Cytologie, cytologie, buněčné interakce, chromozomy, dělení buněk, buněčný cyklus, pohlavní rozmnožování, cytology, cell interaction, chromosomes, cell division, cell cycle, sexual reproduction, 576, 576.54, 576.316, 576.35, 576.36, 576.37, (048.8), and 2
Language:
German
Description:
von F. Vejdovský. and KČSN - S-H 888
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public