The rubber tree (Hevea brasiliensis) is an important tropical crop with a high economic value that has been successfully cultivated in Xishuangbanna, China. Xishuangbanna has a long dry season (November-February) with cold nights and frequent fog events. Thus, it is important to select chilling-tolerant cultivars in order to understand better the role of fog in protecting rubber tree from chilling-induced photodamage. In this study, we examined the photosynthetic responses of six rubber tree cultivars (Lan 873, Yunyan 77-2, Yunyan 77-4, GT1, Reken 523, and Reyan 733-97) to night-chilling stress (0, 5, and 10°C) and two different irradiances (100 and 50% of full sunlight). Our results showed that all six cultivars could withstand nights at 10°C for three days, while night chilling at 0 and 5°C impaired photosynthesis, which was indicated by photoinhibition, decrease of soluble protein content, and accumulation of malondialdehyde. Reken 523 and Reyan 733-97 were more sensitive to night chilling than other cultivars. Low irradiance (50% of full sunlight) after the chilling treatment apparently mitigated the effect of night-chilling stress. It indicates that frequent fog events after cold nights might greatly contribute to the success of rubber tree cultivation in Xishuangbanna., Y.-H. Tian, H.-F. Yuan, J. Xie, J.-W. Deng, X.-S. Dao, Y.-L. Zheng., and Seznam literatury
The generation of superoxide anion radical (O2.-) in the cytochrome b6f complex (Cyt b6f) of spinach under high-light illumination was studied using electron paramagnetic resonance spectroscopy. The generation of O2.- was lost in the absence of molecular oxygen. It was also suppressed in the presence of NaN3 and could be scavenged by extraneous antioxidants such as ascorbate, β-carotene, and glutathione. The results also indicate that O2.-, which is produced under high-light illumination of the Cyt b6f from spinach, might be generated from a reaction involing 1O2, and the Rieske Fe-S protein could serve as the electron donor in the O2.- production. The mechanism of photoprotection of the Cyt b6f complex by antioxidants is discussed. and M. Sang ... [et al.].
This study was aimed to investigate the impact of serine/arginine-rich splicing factor 3 (SRSF3) on the proliferation and migration of gastric cancer (GC) cells. SRSF3 levels in GC tissues and cell lines were measured by Western blotting. Functional assays were used for evaluation of GC cell proliferation, migration and invasion. The PI3K/AKT/mTOR pathway was then examined by Western blotting. SRSF3 exhibits abnormal expression for the significantly increased levels in GC. SRSF3 knockdown significantly suppressed GC progression. SRSF3 knockdown significantly inhibited activation of PI3K/AKT/mTOR signalling. Inhibition of SRSF3 alleviates proliferation and migration of GC cells, and this process is mediated by inactivation of PI3K/ AKT/mTOR signalling. Targeting SRSF3 may be a promising strategy to combat GC.
Monolayer films of phycobilisome-thylakoid membrane complexes isolated from Spirulina platensis were prepared at air/aqueous solution interface by using the Langmuir-Blodgett technique. The film preparation was optimized with 0.5 M phosphate buffer (pH 7.0) as sub-phase at 20 °C. The monolayer was transferred into grids and into mica surface for observing the surface image of the complexes by transmission electron microscopy and atomic force microscope, respectively. The shape of complexes was disk-like with the diameter of about 50 nm and the thickness of about 35 nm. The absorption and fluorescence spectra of the complexes in the monolayer were consistent with those in buffer solution, which suggests that the complexes in the monolayer preserve the basic functional groups of photosynthetic apparatus and can be used as a model to investigate the structural connection and functional association of the light-harvesting antenna with the reaction centres. and D.-H. Li ... [et al.].
A field trial was conducted to determine the effect of nitrogen-enriched biochar on soil water content, plant’s photosynthetic parameters, and grain yield of spring wheat at the Dingxi Experimental Station during the 2014 and 2015 cropping seasons. Results showed that biochar applied with nitrogen fertilizer at a rate of 50 kg ha-1 of N (BN50) increased soil water content in the 0-30 cm depth range by approximately 40, 32, and 53% on average at anthesis, milking, and maturity, respectively, compared with
zero-amendment (CN0). Stomatal conductance and net photosynthetic rate after the BN50 treatment increased by approximately 40 to 50% compared to CN0. Soil water content and photosynthetic traits also increased in other treatments using straw plus nitrogen fertilizer, but to lesser extent than that of BN50. Grain yields were highest (1905 and 2133 kg ha-1 in 2014 and 2015, respectively) under BN50. From this, biochar appears to have a potential for its use with N-fertilizer as a cost-effective amendment for crop production in semiarid environments., S. Yeboah, R. Zhang, L. Cai, L. Li, J. Xie, Z. Luo, J. Wu, D. L. Antille., and Obsahuje bibliografii