Handy Plant Efficiency Analyser (Handy PEA) provides a method for the high-throughput screening of photosynthetic germplasm. However, the large number of chlorophyll a fluorescence parameters (CFPs) from PEA and the inconsistency of CFP applications among studies greatly limit the accuracy of photosynthesis analyses. In this study, all 53 CFPs of 186 upland cotton cultivars (strains) were measured at 12:00 and 17:00 h. Thirty-two CFPs were selected according to biological importance, and the CFP relationships were determined. Differences in the response ability of cotton cultivars (strains) to high light intensity stress were demonstrated by the distribution of CFPs. Furthermore, the classification and evaluation of photosynthetic characteristics of cotton cultivars (strains) were carried out by Principal Component Analysis and Cluster Analysis. Finally, ten cotton cultivars (strains) with good photosynthetic performance were selected. This study provides a high-throughput method how to identify cotton germplasm resources with high photosynthetic efficiency.
Melatonin mediates many physiological processes in animals and plants. To examine the potential roles of melatonin in salinity tolerance, we investigated the effects of exogenous melatonin on growth and antioxidant system in cucumber under 200 mM NaCl stress conditions. The results showed that the melatonin-treated plants significantly increased growth mass and antioxidant protection. Under salinity stress, the addition of melatonin effectively alleviated the decrease in the net photosynthetic rate, the maximum quantum efficiency of PSII, and the total chlorophyll content. Our data also suggested that melatonin and the resistance of plants exhibited a concentration effect. The application of 50-150 μM melatonin significantly improved the photosynthetic capacity. Additionally, the pretreatment with melatonin reduced the oxidative damage under salinity stress by scavenging directly H2O2 or enhancing activity of antioxidant enzymes (including superoxide dismutase, peroxidase, catalase, ascorbate peroxidase) and concentrations of antioxidants (ascorbic acid and glutathione). Therefore, the melatonin-treated plants could effectively enhance their salinity tolerance., L. Y. Wang, J. L. Liu, W. X. Wang, Y. Sun., and Obsahuje seznam literatury
A total genome scan and pharmacogenetic study were designed to search for genetic determinants of blood pressure (BP) as well as heart and kidney weights. Genome scanning was carried out in 266 F2 intercrosses from Prague hypertensive hypertriglyceridemic rats for phenotypes of organ weights, baseline BP, BP after blockade of the renin-angiotensin system (RAS) by losartan, of the sympathetic nervous system (SNS) by pentolinium, and of the nitric oxide (NO) synthase by NG-nitro-L-arginine methyl ester. Pharmacogenetic analysis showed that, in males, BP was controlled by two loci on chromosomes 1 and 5 (Chr1, Chr5) through the SNS, and these loci showed a positive contribution for relative kidney weight (KW/BW). On the other hand, baseline BP in females was controlled by two loci on Chr3 and Chr7. The effect of these loci was not mediated by the RAS, SNS or NO system. These loci did not show any effect for KW/BW. Negatively-linked loci for KW/BW and relative heart weight (HW/BW) were identified on Chr2 in both genders. Another negatively-linked locus for KW/BW, located on Chr8 in males, affected BP through the SNS. This locus on Chr8 overlapped with a previously-reported modifier locus for polycystic kidney disease (PKD). In conclusion, this pharmacogenetic study determined two loci for BP and relative organ mass implicating sympathetic overactivity. Concordance of the identified locus for KW/BW and BP through the SNS on Chr8 with the PKD locus revealed the importance of this region for renal complications in various diseases., T. Ueno, J. Tremblay, J. Kuneš, J. Zicha, Z. Dobešová, Z. Pausová, A. Y. Deng, Y. Sun, H. J. Jacob, P. Hamet., and Obsahuje bibliografii
This study was aimed to investigate the impact of serine/arginine-rich splicing factor 3 (SRSF3) on the proliferation and migration of gastric cancer (GC) cells. SRSF3 levels in GC tissues and cell lines were measured by Western blotting. Functional assays were used for evaluation of GC cell proliferation, migration and invasion. The PI3K/AKT/mTOR pathway was then examined by Western blotting. SRSF3 exhibits abnormal expression for the significantly increased levels in GC. SRSF3 knockdown significantly suppressed GC progression. SRSF3 knockdown significantly inhibited activation of PI3K/AKT/mTOR signalling. Inhibition of SRSF3 alleviates proliferation and migration of GC cells, and this process is mediated by inactivation of PI3K/ AKT/mTOR signalling. Targeting SRSF3 may be a promising strategy to combat GC.