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Start Over Creator Jana Juhásová

1. Composite hyaluronate-type I collagen-fibrin scaffold in the therapy of osteochondral defects in miniature pigs

Creator:
Elena Filová, Michala Rampichová, Milan Handl, Lytvynets, A., Roman Halouzka, Dušan Usvald, Jana Juhásová, Radek Procházka, Monika Dezortová, Eva Košťáková, Tomáš Trč, Eduard Šťastný, Koláčná, L., Milan Hájek, Jan Motlík, and Evžen Amler
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, osteochondral defect, fibrin, autologous chondrocytes, cartilage regeneration, 14, and 612
Language:
English
Description:
E. Filová, M. Rampichová, M. Handl, A. Lytvynets, R. Halouzka, D. Usvald, J. Hlučilová, R. Procházka, M. Dezortová, E. Rolencová, E. Košťáková, T. Trč, E. Šťastný, L. Koláčná, M. Hájek, J. Motlík, E. Amler. and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Osteogenic differentiation of miniature pig mesenchymal stem cells in 2D and 3D environment

Creator:
Jana Juhásová, Štefan Juhás, Jiří Klíma, Ján Strnádel, Holubová, M., and Jan Motlík
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, diferenciace buněk, popraviště, cell differentiation, scaffolds, mesenchymal stem cells, miniature pig, 14, and 612
Language:
English
Description:
Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteog enic differentiation of miniature pig MSCs and markers of this differentiation in vitro . Flow-cytometrically characterized MSCs were seeded on cultivation plastic (collagen I and vitronectin coated/uncoated) or plasma clot (PC)/plasma- alginate clot (PAC) scaffolds and differentiated in osteogenic medium. During three weeks of differentiation, the formation of nodules and deposition of calcium were visualized by Alizarin Red Staining. In addition, the production of alkaline phosphatase (ALP) activity was quantitatively detected by fluorescence. The expression of osteopontin, osteonectin and osteocalcin were assayed by immunohistochemistry and Western Blot analysis. We revealed a decrease of osteopontin expression in 2D and 3D environment during differentiation. The weak initial osteonectin signal, culminating on 7th or 14th day of differentiation, depends on collagen I and vitronectin coating in 2D system. The highest activity of ALP was detected on 21th day of osteogenic differentiation. The PC scaffolds provided better conditions for osteogenic differentiation of MSCs than PAC scaffolds in vitro . We also observed expected effects of collagen I and vitronectin on the acceleration of osteogenic differentiation of miniature pig MSC. Our results indicate similar ability of miniature pig MSCs osteogenic differentiation in 2D and 3D environment, but the expression of osteogenic marker s in scaffolds and ECM coated monolayers started earlier than in the monolayers without ECM., J. Juhásová ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Quality of newly formed cartilaginous tissue in defects of acticular surface after transplantation of mesenchymal stem cells in a composite scaffold based on collagen I with chitosan micro- and nanofibres

Creator:
Alois Nečas, Ladislav Plánka, Robert Srnec, Michal Crha, Jana Juhásová, Jiří Klíma, David Starý, Leoš Křen, Evžen Amler, Vojtová, L., Josef Jančář, and Petr Gál
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, kloubní chrupavka, articular cartilage, bone marrow mesenchymal stem cells, allogeneic, micropicking, 14, and 612
Language:
English
Description:
The aim of this study was to evaluate macroscopically, histologically and immunohistochemically the quality of newly formed tissue in iatrogenic defects of articular cartilage of the femur condyle in miniature pigs treated with the clinically used method of microfractures in comparison with the transplantation of a combination of a composite scaffold with allogeneic mesenchymal stem cells (MSCs) or the composite scaffold alone. The newly formed cartilaginous tissue filling the defects of articular cartilage after transplantation of the scaffold with MSCs (Group A) had in 60 % of cases a macroscopically smooth surface. In all lesions after the transplantation of the scaffold alone (Group B) or after the method of microfractures (Group C), erosions/fissures or osteophytes were found on the surface. The results of histological and immunohistochemical examination using the modified scoring system according to O’Driscoll were as follows: 14.7±3.82 points after transplantations of the scaffold with MSCs (Group A); 5.3±2.88 points after transplantations of the scaffold alone (Group B); and 5.2±0.64 points after treatment with microfractures (Group C). The O’Driscoll score in animals of Group A was significantly higher than in animals of Group B or Group C (p<0.0005 both). No significant difference was found in the O’Driscoll score between Groups B and C. The treatment of iatrogenic lesions of the articular cartilage surface on the condyles of femur in miniature pigs using transplantation of MSCs in the composite scaffold led to the filling of defects by a tissue of the appearance of hyaline cartilage. Lesions treated by implantation of the scaffold alone or by the method of microfractures were filled with fibrous cartilage with worse macroscopic, histological and immunohistochemial indicators., A. Nečas ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs

Creator:
Ladislav Plánka, Alois Nečas, Robert Srnec, Petr Raušer, David Starý, Josef Jančář, Evžen Amler, Eva Filová, Jana Juhásová, Leoš Křen, and Petr Gál
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie, poranění, physiology, wounds and injuries, růstová ploténka, fysis, deformace končetiny, mesenchymální kmenové buňky, growth plate, physis, limb deformity, mesenchymal stem cells, 14, and 612
Language:
English
Description:
This study appears from an experiment previously carried out in New Zealand white rabbits. Allogenic mesenchymal stem cells (MSCs) were transplanted into an iatrogenically-created defect in the lateral section of the distal physis of the left femur in 10 miniature pigs. The right femur with the same defect served as a control. To transfer MSCs, a freshly prepared porous scaffold was used, based on collagen and chitosan, constituting a compact tube into which MSCs were implanted. The pigs were euthanized four months after the transplantation. On average, the left femur with transplanted MSCs grew more in length (0.56±0.14 cm) compared with right femurs with physeal defect without transplanted MSCs (0.14 ± 0.3 cm). The average angular (valgus) deformity of the left femur had an angle point of 0.78°, following measurement and X-ray examination, whereas in the right femur without transplantation it was 3.7°. The initial results indicate that preventive transplantation of MSCs into a physeal defect may prevent valgus deformity formation and probably also reduce disorders of the longitudinal bone growth. This part of our experiment is significant in the effort to advance MSCs application in human medicine by using pig as a model, which is the next step after experimenting on rabbits., L. Plánka ... [et al.]., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public