Induced pluripotent stem cells (iPSC) are undifferentiated cells capable of unlimited self-renewal. By introducing specific transcription factors, any somatic (fully differentiated) cell of the mammalian body can be changed (reprogrammed) into iPSC. The iPSCs are very similar to embryonic stem cells in their molecular and functional properties. By using specific factors, iPSCs can be differentiated into each of the about 200 cell types in the human body. The big advantage of iPSCs is the availability of somatic cells (isolation from blood or skin biopsy). Creation of "tailor made" iPSCs for each patient would allow transplantation of own cells and avoid problems with finding an immunologically compatible donor. and Kateřina Vodičková Kepková, Petr Vodička, Jan Motlík.
E. Filová, M. Rampichová, M. Handl, A. Lytvynets, R. Halouzka, D. Usvald, J. Hlučilová, R. Procházka, M. Dezortová, E. Rolencová, E. Košťáková, T. Trč, E. Šťastný, L. Koláčná, M. Hájek, J. Motlík, E. Amler. and Obsahuje bibliografii
The glycophenotyping of mammalian cells with plant lectins maps aspects of the glycomic profile and disease-associated alterations. A salient step toward delineating their functional dimension is the detection of endogenous lectins. They can translate sugar-encoded changes into cellular responses. Among them, the members of the lectin family of galectins are emerging regulators of cell adhesion, migration and proliferation. Focusing on galectins-1, -3 and -7, we addressed the issue whether their expression is regulated during wound healing in porcine skin as model. A conspicuous upregulation is detected for galectin-1 in the dermis and a neoexpression in the epidermis, where an increased level of galectin-7 was also found. Applying biotinylated tissue lectins as probes, the signal intensities for accessible binding sites decreased, intimating an interaction of the cell lectin with reactive sites. In contrast, galectin-3 parameters remained rather constant. Of note, epidermal cells in culture also showed an increase in expression/presence of galectin-1, measured on the levels of mRNA and protein, in this case by Western blotting and quantitative immunocytochemistry. Used as matrix, galectin-1 conferred resistance to trypsin treatment to attached human keratinocytes and reduced migration into scratch-wound areas in vitro. This report thus presents new information on endogenous lectins in wound healing and differential regulation among the three tested cases., J. Klíma ... [et al.]., and Obsahuje seznam literatury
Cílená změna původní identity buňky v identitu jinou neboli reprogramování buněk nachází své uplatnění jak v základním výzkumu, tak i v případné buněčné terapii. Z mnoha postupů reprogramování autoři představují přípravu indukovaných pluripotentních buněk (iPS), transdiferencovaných anebo přímo reprogramovaných buněk. Je popsána cesta přípravy a z ní plynoucí vlastnosti a potenciál indukovaných buněk., Induced change in the identity of the cell known as reprogramming of the somatic cell is applicable both in basic research and cell therapy. Out of many reprogramming approaches the authors introduce the derivation of induced pluripotent stem (iPS), transdifferentiated and direct reprogrammed cells. The workflow for the preparation of induced cells and a comment on their properties and potentials are given., and Jiří Klíma, Irena Lišková, Jan Motlík.
Když se mezinárodní organizační výbor rozhodoval, kde uspořádá konferenci The 2nd Large Animal Models of Neurodegenerative Diseases, nikdo nepochyboval, že se chce vrátit na místo konference první - zámek Liblice. Vysoký standard služeb v Konferenčním centru AV ČR a prostředí zámku, které tvoří jedinečný rámec jak pro vědecká jednání, tak osobní setkání, představují pro naši vědeckou komunitu jedinečnou devizu. and Jan Motlík.
Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteog enic differentiation of miniature pig MSCs and markers of this differentiation in vitro . Flow-cytometrically characterized MSCs were seeded on cultivation plastic (collagen I and vitronectin coated/uncoated) or plasma clot (PC)/plasma- alginate clot (PAC) scaffolds and differentiated in osteogenic medium. During three weeks of differentiation, the formation of nodules and deposition of calcium were visualized by Alizarin Red Staining. In addition, the production of alkaline phosphatase (ALP) activity was quantitatively detected by fluorescence. The expression of osteopontin, osteonectin and osteocalcin were assayed by immunohistochemistry and Western Blot analysis. We revealed a decrease of osteopontin expression in 2D and 3D environment during differentiation. The weak initial osteonectin signal, culminating on 7th or 14th day of differentiation, depends on collagen I and vitronectin coating in 2D system. The highest activity of ALP was detected on 21th day of osteogenic differentiation. The PC scaffolds provided better conditions for osteogenic differentiation of MSCs than PAC scaffolds in vitro . We also observed expected effects of collagen I and vitronectin on the acceleration of osteogenic differentiation of miniature pig MSC. Our results indicate similar ability of miniature pig MSCs osteogenic differentiation in 2D and 3D environment, but the expression of osteogenic marker s in scaffolds and ECM coated monolayers started earlier than in the monolayers without ECM., J. Juhásová ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy