Differential regulation of galectin expression/reactivity during wound healing in porcine skin and in culture of epidermal cells with functional impact of migration

Title:

Differential regulation of galectin expression/reactivity during wound healing in porcine skin and in culture of epidermal cells with functional impact of migration

Creator:

Jiří Klíma, Lukáš Lacina, Barbora Dvořánková, Herrmann, D., Carnwath, J. W., Niemann, H., Kaltner, H., Sabine André, Jan Motlík, Hans-Joachim Gabius, and Karel Smetana

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:84f8c0d9-72b9-4171-a24b-45c297616eb5
uuid:84f8c0d9-72b9-4171-a24b-45c297616eb5

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, epidermis, kůže (anatomie), hojení ran, physiology, skin, wound healing, keratinocyty, lektin, keratinocytes, lectin, 14, and 612

Type:

article, články, model:article, and TEXT

Description:

The glycophenotyping of mammalian cells with plant lectins maps aspects of the glycomic profile and disease-associated alterations. A salient step toward delineating their functional dimension is the detection of endogenous lectins. They can translate sugar-encoded changes into cellular responses. Among them, the members of the lectin family of galectins are emerging regulators of cell adhesion, migration and proliferation. Focusing on galectins-1, -3 and -7, we addressed the issue whether their expression is regulated during wound healing in porcine skin as model. A conspicuous upregulation is detected for galectin-1 in the dermis and a neoexpression in the epidermis, where an increased level of galectin-7 was also found. Applying biotinylated tissue lectins as probes, the signal intensities for accessible binding sites decreased, intimating an interaction of the cell lectin with reactive sites. In contrast, galectin-3 parameters remained rather constant. Of note, epidermal cells in culture also showed an increase in expression/presence of galectin-1, measured on the levels of mRNA and protein, in this case by Western blotting and quantitative immunocytochemistry. Used as matrix, galectin-1 conferred resistance to trypsin treatment to attached human keratinocytes and reduced migration into scratch-wound areas in vitro. This report thus presents new information on endogenous lectins in wound healing and differential regulation among the three tested cases., J. Klíma ... [et al.]., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2009 Volume:58 | Number:6

Harvested from:

CDK

Metadata only:

false