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Start Over Subject HL-60 cells

1. Apoptotic damage of DNA in Hhuman leukaemic HL-60 cells treated with C2-ceramide was detected after G1 blockade of the cell cycle

Creator:
Bártová, E., Španová, A., Janáková, L., Bobková, M., and Rittich, B.
Type:
article, model:article, and TEXT
Subject:
HL-60 cells, C2-ceramide, apoptosis, and cell cycle
Language:
English
Description:
Apoptosis was induced by treatment of HL-60 cells with C2-ceramide. Apoptotic damage of DNA was detected according to the sub-Gl peak on a flow cytometer, according to the typical morphology and according to the DNA fragmentation "ladder" after gel electrophoresis. It was shown that the apoptotic cleavage followed after G1 blockade of the cell cycle. A high correlation coefficient (rs=0.957) was found between the percentages of G1 blocked cells and apoptotic cells. This high correlation together with the appearance of the sub-Gl peak suggests that the G1 blocked HL-60 cells were subject to apoptotic death. It is deduced that the mechanisms leading to G1 blockade of the cell cycle and activation of apoptosis in HL-60 cells are interconnected.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Modulation of ionizing radiation-induced apoptosis and cell cycle arrest by all-trans retinoic acid in promyelocytic leukemia cells (HL-60)

Creator:
Mária Mareková, Jaroslava Vávrová, Doris Vokurková, and Jan Psutka
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, apoptóza, ionizující záření, apoptosis, ionizing radiation, all-trans retinoic acid, HL-60 cells, 14, and 612
Language:
English
Description:
Acute promyelocytic leukemia is characterized by a block of myeloid differentiation. The incubation of cells with 1 μmol/l all-trans retinoic acid (ATRA) for 72 h induced differentiation of HL-60 cells and increased the number of CD11b positive cells. Morphological and functional changes were accompanied by a loss of proliferative capacity. Differentiation caused by preincubation of leukemic cells HL-60 with ATRA is accompanied by loss of clonogenicity (control cells: 870 colonies/103 cells, cells preincubated with ATRA: 150 colonies/103 cells). D0 for undifferentiated cells was 2.35 Gy, for ATRA differentiated cells 2.46 Gy. Statistical comparison of clonogenity curves indicated that in the whole range 0.5-10 Gy the curves are not significantly different, however, in the range 0.5-3 Gy ATRA differentiated cells were significantly more radioresistant than non-differentiated cells. When HL-60 cells preincubated with 1 μmol/l ATRA were irradiated by a sublethal dose of 6 Gy, more marked increase of apoptotic cells number was observed 24 h after irradiation and the surviving cells were mainly in the G1 phase of the cell cycle, while only irradiated cells were accumulated in G2 phase. Our results imply that preincubation of cells with ATRA accelerates apoptosis occurrence (24 h) after irradiation by high sublethal dose of 6 Gy. Forty-eight hours after 6 Gy irradiation, late apoptotic cells were found in the group of ATRA pretreated cells, as determined by APO2.7 positivity. This test showed an increased effect (considering cell death induction) in comparison to ATRA or irradiation itself., M. Mareková, J. Vávrová, D. Vokurková, J. Psutka., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public