To date, thousands of microRNAs (miRNAs) and their precursors (pre-miRNAs) have been identified in insects and their nucleotide sequences deposited in the miRBase database. In the present work, we have systematically analyzed, utilizing bioinformatics tools, the featural differences between human and insect pre-miRNAs, as well as differences across 24 insect species. Results showed that the nucleotide composition, sequence length, nucleotides preference and secondary structure features between human and insects were different. Subsequently, with the aid of three available SVM-based prediction programs, pre-miRNA sequences were evaluated and given corresponding scores. Thus it was found that of 2633 sequences from the 24 chosen insect species, 2229 (84.7%) were successfully recognized by the Mirident classifier, higher than Triplet-SVM (72.5%) and PMirP (72.6%). In contrast, four species, including the domesticated silkworm, Bombyx mori L., the fruit fly, Drosophila melanogaster Meigen, the honeybee, Apis mellifera L. and the red flour beetle, Tribolium castaneum (Herbst), were found to be largely responsible for the poor performance of some sequence matching. Compared with other species, B. mori especially showed the worst performance with the lowest average MFE index (0.73). Collectively these results pave the way for understanding specificity and diversity of miRNA precursors in insects, and lay the foundation for the further development of more suitable algorisms for insects., Li, Jisheng ... [et al.]., and Obsahuje seznam literatury
Současná biologie se bouřlivě rozvíjí v oblasti genomiky. Tyto přístupy pronikají stále více do ekologie i evoluční biologie. Vznikl nový obor populační genomika, který propojuje terénní a laboratorní biologii. Využívá nové metodiky sekvenování a bioinformatických analýz, které v tomto článku stručně popisujeme a vysvětlujeme., Genomics is a rapidly developing field of modern biology. Novel genomic approaches are increasingly utilized in ecology and evolutionary biology. Population genomics has been recently established as a new discipline, connecting field and laboratory biology. It uses new sequencing methods as well as bioinformatic analyses, briefly described and explained in this article., and Helena Štorchová.
The purpose of the workshop, which took place in 8-15 June 2008, was to train scientists to manage and exploit large EST datasets as well as compare whole genome sequences, with the aim of faciliting the application of genomic technologies to ticks and tickbornepathogens. The course provided an overview of the mehods used to sequence, assemble, and annotate tick borne disease related genomes and EST datasets. and Libor Grubhoffer.
22nd Annual Mammalian Genome Conference took place in the new edifice of the Institute of Molecular Chemistry AS CR, v. v. i., in Prague. The symposium was aimed at dissiminating research news on the topic mammalian genomes, epigenomes and phenotypes. It began with Bioinformatics speakers folowed by a Student Satellite Meeting and it continued with a number of sessions. and Jiří Forejt.