Levels of conjugated dienes of fatty acids (first peroxidation product) in relation to their substrates and promotors (triacylglycerols, homocysteine, iron) as well as to their inhibitors (essential antioxidative vitamins) were assessed in a vegetarian group (n=24) and compared with subjects on a mixed diet (traditional nutrition, n=24). Positive significant linear correlation between conjugated dienes and triacylglycerols, homocysteine, iron as well as inverse relationship between conjugated dienes and vitamin E, vitamin C, beta-carotene were observed in pooled groups. Lipid peroxidation risk in vegetarians seems to be caused predominantly by hyperhomocysteinemia, whereas in a mixed diet group this was due to a higher supply of substrates or risk iron values. The incidence of only 8 % of risk conjugated diene values in vegetarians in contrast to 42 % in the group with traditional diet indicates that vegetarians have a better antioxidative status as a consequence of regular consumption of protective food.
a1_The decreased oxidizability of plasma lipoproteins is related to the increased vitamin E intake and its association with a relatively lower incidence of coronary heart disease has been proposed. We investigated the effect of the in vivo vitamin E supplementation on the oxidizability of serum lipids in patients with ischemic heart disease and a moderate hypercholesterolemia. Thirty-two patients (16 males and 16 postmenopausal women) participated in this placebo-controlled, randomized trial. They were treated with 400 mg vitamin E/day for 6 weeks. The copper-induced serum lipid oxidizability ex vivo was assessed by measuring conjugated diene formation at 245 nm. We also measured vitamin E, malondialdehyde (MDA) and uric acid concentrations in the plasma. Because of observed significant differences in parameters of serum lipid oxidizability (lag time and maximal rate of oxidation), plasma a-tocopherol and MDA levels between male patients and postmenopausal women supplemented with vitamin E, the results were compared between both genders. Six weeks of vitamin E supplementation significantly increased plasma vitamin E levels (by 87 %) in male patients but in postmenopausal women only by 34 %. Concomitantly with increased plasma levels of vitamin E the decrease in plasma MDA levels was observed in male patients (decrease by 20 %; p=0.008), but in postmenopausal women the decrease did not attain statistical significance. Plasma uric acid levels were not apparently changed in placebo or vitamin E supplemented groups of patients. The changes in ex vivo serum lipid oxidizability after vitamin E, supplementation have shown a significantly prolonged lag time (by 11 %; p=0.048) and lowered rate of lipid oxidation (by 21 %; p=0.004) in male patients in comparison with postmenopausal women., a2_Linear regression analysis revealed a significant correlation between plasma vitamin E levels and the lag time (r=0.77; p=0.03) and the maximal rate of serum lipid oxidation (r=-0.70; p=0.05) in male patients. However, in postmenopausal women the correlations were not significant. We conclude that 400 mg vitamin E/day supplementation in patients with ischemic heart disease and a moderate hypercholesterolemia influenced favorably ex vivo serum lipid oxidation of male patients when compared with postmenopausal women. The observed differences between both genders could be useful in the selection of the effective vitamin E doses in the prevention of coronary heart disease., A. Nagyová, V. Mongiellová, Z. Krivošíková, P. Blažíček, V. Spustová, M. Gajdoš, R. Dzúrik., and Obsahuje bibliografii
Current study evaluated the synergistic potential of propolis and
vitamin E against sub-acute toxicity of aluminum chloride on
different biochemical parameters and liver histology. Swiss albino
mice (n=42) were randomly divided into seven groups. Group I
received 0.2 ml of 0.9 % saline solution, Group II received
Propolis (50 mg/kg b.w.), Group III received vitamin E
(150 mg/kg b.w.), Group IV received AlCl3 50 mg/kg b.w.,
Group V received AlCl3 + Propolis, Group VI received AlCl3 +
vitamin E and Group VII received AlCl3 + propolis + vitamin E.
Blood and tissue samples were collected after 7 and 21 days. The
body weight of the animals significantly increased in all groups
except Group IV. The concentration of serum high density
lipoprotein significantly decreased in Group IV and increased in
Group V, VI and VII. The level of aspartate aminotransferase,
alanine transferase, alkaline phosphatase, triglycerides, total
cholesterol, and low density lipoprotein significantly increased in
AlCl3 treated group and increased in Group V, VI and VII. Tissue
sections were processed and stained by hematoxylin and eosin.
Group II showed cellular necrosis. Group V, VI showed decreased
number of vacuolization, sinusoidal spacing and macrophage cell
infiltration. Group VI showed less degenerative changes in the
third week. Vitamin E and propolis in combination with Al
provides more protection against AlCl3 induced toxicity.