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Start Over Creator Spustová, V.

2. Lipid Peroxidation and Nutrition

Creator:
Krajčovičová-Kudláčková, M., Spustová, V., and Pauková, V.
Type:
article, model:article, and TEXT
Subject:
Conjugated dienes of fatty acids, Homocysteine, Vitamin C, and Vitamin E
Language:
English
Description:
Levels of conjugated dienes of fatty acids (first peroxidation product) in relation to their substrates and promotors (triacylglycerols, homocysteine, iron) as well as to their inhibitors (essential antioxidative vitamins) were assessed in a vegetarian group (n=24) and compared with subjects on a mixed diet (traditional nutrition, n=24). Positive significant linear correlation between conjugated dienes and triacylglycerols, homocysteine, iron as well as inverse relationship between conjugated dienes and vitamin E, vitamin C, beta-carotene were observed in pooled groups. Lipid peroxidation risk in vegetarians seems to be caused predominantly by hyperhomocysteinemia, whereas in a mixed diet group this was due to a higher supply of substrates or risk iron values. The incidence of only 8 % of risk conjugated diene values in vegetarians in contrast to 42 % in the group with traditional diet indicates that vegetarians have a better antioxidative status as a consequence of regular consumption of protective food.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Participation of P-dependent and P-independent glutaminases in rat kidney ammoniagenesis and their modulation by metabolic acidosis, hippurate and insulin

Creator:
Krivošíková, Z., Spustová, V., and Dzúrik, R.
Type:
article, model:article, and TEXT
Subject:
ammoniagenesis, P-independent glutaminase, P-dependent glutaminase, metabolic acidosis, hippurate, and insulin
Language:
English
Description:
The key regulatory enzymes of kidney ammoniagenesis appear to be P-dependent (PDG) and P-independent (PIG) glutaminases. While the participation of PDG has been satisfactorily elucidated, the significance of PIG remains doubtful. Rat kidney cortex slices synthesized ammonia even under basal conditions. Metabolic acidosis, hippurate and insulin stimulated ammonia production. Under basal conditions, PDG activity in kidney homogenate, was twice as high as PIG activity. Metabolic acidosis stimulated ammonia production by the stimulation of both PDG (100 %) and PIG (57 %) activities. Hippurate stimulated only PIG activity both under basal conditions (90 %) and in metabolic acidosis (52 %), while it inhibited PDG activity only insignificantly under basal conditions and markedly (53 %) in metabolic acidosis. Insulin stimulated both PIG and PDG activities under basal conditions as well as in metabolic acidosis and potentiated the PIG stimulation by hippurate while it potentiated the hippurate inhibition of PDG both under basal conditions and in acidotic rats. In conclusion, both PDG and PIG participate in ammoniagenesis and are stimulated by metabolic acidosis and insulin. Hippurate stimulates PIG, while it inhibits PDG in metabolic acidosis and even after insulin administration. The effect of hippurate appears to be of physiological interest.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public