Current study evaluated the synergistic potential of propolis and
vitamin E against sub-acute toxicity of aluminum chloride on
different biochemical parameters and liver histology. Swiss albino
mice (n=42) were randomly divided into seven groups. Group I
received 0.2 ml of 0.9 % saline solution, Group II received
Propolis (50 mg/kg b.w.), Group III received vitamin E
(150 mg/kg b.w.), Group IV received AlCl3 50 mg/kg b.w.,
Group V received AlCl3 + Propolis, Group VI received AlCl3 +
vitamin E and Group VII received AlCl3 + propolis + vitamin E.
Blood and tissue samples were collected after 7 and 21 days. The
body weight of the animals significantly increased in all groups
except Group IV. The concentration of serum high density
lipoprotein significantly decreased in Group IV and increased in
Group V, VI and VII. The level of aspartate aminotransferase,
alanine transferase, alkaline phosphatase, triglycerides, total
cholesterol, and low density lipoprotein significantly increased in
AlCl3 treated group and increased in Group V, VI and VII. Tissue
sections were processed and stained by hematoxylin and eosin.
Group II showed cellular necrosis. Group V, VI showed decreased
number of vacuolization, sinusoidal spacing and macrophage cell
infiltration. Group VI showed less degenerative changes in the
third week. Vitamin E and propolis in combination with Al
provides more protection against AlCl3 induced toxicity.