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12. 4th CCP Phenogenomics Conference 2022: abstract book: hybrid conference: Vestec, 15-16 September 2022
- Creator:
- Ústav molekulární genetiky (Akademie věd ČR), České centrum pro fenogenomiku, and CCP Phenogenomics Conference (2022 : Vestec, Praha-západ, Česko)
- Publisher:
- Institute of Molecular Genetics of the Czech Academy of Sciences
- Format:
- electronic, electronic resource, remote, bez média, online zdroj, and 82 stran : ilustrace, převážně barevné, portréty
- Type:
- model:monograph and TEXT
- Subject:
- Obecná genetika. Obecná cytogenetika. Evoluce, fenogenomika, molekulární genetika, phenogenomics, molecular genetics, 575.111:575.21, 577.21, (062.534), (048.3), 2, and 575
- Language:
- English
- Description:
- Název z obálky and born digital
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
13. 6-Hydroxydopamine-induced neurotoxicity in rat model of Parkinson’s disease: is reversed via anti-oxidative activities of curcumin and aerobic exercise therapy
- Creator:
- Sokouti, Hadis, Mohajeri, Daryoush, and Nourazar, Mir Alireza
- Format:
- počítač and online zdroj
- Type:
- model:article and TEXT
- Subject:
- 6-OHDA, Parkinson's disease, aerobic exercise, curcumin, behavioral tasks, and oxidative stress
- Language:
- English
- Description:
- In the rat model, 6-hydroxydopamine (6-OHDA) known as a selective catecholaminergic neurotoxin used chiefly in modeling Parkinson's disease (PD). Continuous aerobic exercise and curcumin supplementations could play a vital role in neuroprotection. This study aimed to explore the neuroprotective roles of regular aerobic exercise and curcumin during PD. For this, rats were treated as follows for 8 consecutive weeks (5 d in a week): For this, animals were orally treated with curcumin (50 ml/kg) alone or in combination with aerobic exercise. Compared with a control group, induction of PD by 6-OHDA increased the amount of α-synuclein protein and malondialdehyde levels and decreased the number of substantia nigra neurons, total antioxidant capacity, and glutathione peroxidase activity in brain tissue. All these changes were abolished by the administration of curcumin with aerobic exercise treatments. Activity behavioral tests also confirmed the above-mentioned results by increasing the rod test time and the number of rotations due to apomorphine injection. Histopathology assays mimic the antioxidant activity and behavioral observations. Combined curcumin with aerobic exercise treatments is potentially an effective strategy for modifying the dopaminergic neuron dysfunction in 6-OHDA-induced rats modeling PD via dual inhibiting oxidative stress indices and regulating behavioral tasks.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
14. 8th International Symposium on Kallikreins and Kallikrein-Related Peptidases: Prague, 25-27 September 2019 : final programme & abstract book
- Creator:
- Ústav molekulární genetiky (Akademie věd ČR) and International Symposium on Kallikreins and Kallikrein-Related Peptidases 2019 : Praha, Česko)
- Publisher:
- Institute of Molecular Genetics CAS
- Format:
- electronic, electronic resource, remote, bez média, online zdroj, and 78 stran
- Type:
- model:monograph and TEXT
- Subject:
- Biochemie. Molekulární biologie. Biofyzika, molekulární genetika, enzymy, molecular genetics, enzymes, kallikrein, 577.21, 577.15, 612.015.1, 615.355, (048.3), (062.534), 2, and 577
- Language:
- English
- Description:
- Název z obálky, Obsahuje bibliografii a bibliografické odkazy, and born digital
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
15. A "clean" alien species? Parasites of the invasive ladybird Harmonia axyridis (Coleoptera: Coccinellidae)
- Creator:
- Dudek, Kryzstof, Sienkiewicz, Paweł, Gwiazdowicz, Dariusz J., and Piotr Tryjanowski
- Format:
- print, počítač, and online zdroj
- Type:
- article, články, journal articles, model:article, and TEXT
- Subject:
- Zoologie, brouci, slunéčkovití, slunéčko východní, hlístice, beetles, Coccinellidae, Harmonia axyridis, Nematodes, phoretic mites, insect ecology, eugregarines, fungi, 2, and 59
- Language:
- English
- Description:
- The multicoloured Asian ladybird Harmonia axyridis is an invasive insect that can negatively influence biodiversity and human economy in invaded areas. According to the enemy release hypothesis, invasive alien species are often little affected by parasites and other enemies. We studied the prevalence of common parasites of insects infesting and infecting H. axyridis in NW Poland. A large sample of 2351 individuals was collected and divided into two groups: 1180 beetles were dissected and examined for the presence of eugregarines, nematodes and Laboulbeniales fungi, and 751 were checked for phoretic mites. Our results show that H. axyridis is indeed parasitized infrequently. The prevalence of eugregarines and nematodes was very low (1.5% and 0.4%, respectively). No specimens of Laboulbeniales or phoretic mites were found. Our study indicates that in NW Poland H. axyridis is rarely infested or infected by parasites. This paper reports for the first time the infection of H. axyridis by the eugregarine Gregarina barbarara., Kryzstof Dudek, Paweł Sienkiewicz, Dariusz J. Gwiazdowicz, Piotr Tryjanowski., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
16. A case study in canine detection of giant bullfrog scent
- Creator:
- Matthew, Esther E., Verster, Ruhan, and Weldon, Ché
- Format:
- počítač and online zdroj
- Type:
- model:article and TEXT
- Subject:
- anurans, improved location, olfaction, positive reinforcement, and sniffer dog
- Language:
- English
- Description:
- Accurate survey methods are required for any wildlife research to yield reliable population data. This constraint finds significance in amphibian research that involves a highly threatened group of animals with a large proportion of cryptic species not easily detected by conventional survey methods. Across a growing spectrum of zoology research, survey outcomes are benefitting from the efficacy of scent detection dogs in assisting with species detection. We investigated the ability of a scent detection dog to locate and identify traces of giant bullfrog, Pyxicephalus adspersus scent and investigate methods of preserving frog scent for use in subsequent conditioning training of dogs. The scent detection dog was able to detect 100,000 times diluted scent with 87% sensitivity and 84% efficacy. High specificity (98,6%) was also achieved while presented with the challenge of detecting P. adspersus scent amid that of other frog species. Detection sensitivity was negatively correlated with scent preservation time but yielded the highest sensitivity for samples that were preserved as skin swabs stored at 4 °C and diluted shortly before use. Conservationists, scientists, and customs officials alike can benefit from scent detection dog detection of amphibians through enhanced sample acquisition rates with reduced collection biases.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
17. A case study of Heleomyzidae (Diptera) recorded on snow in Poland with a review of their winter activity in Europe
- Creator:
- Soszńska-Maj, Agnieszka and Woźnica, Andrzej J.
- Format:
- print, počítač, and online zdroj
- Type:
- article, články, journal articles, model:article, and TEXT
- Subject:
- Zoologie, dvoukřídlí, fenologie, Diptera, phenology, Evropa, Polsko, Europe, Poland, Heleomyzidae, winter activity, snow fauna, 2, and 59
- Language:
- English
- Description:
- Twenty eight species of winter-active Heleomyzidae were collected during a long-term study in Poland. More than 130 samples of insects, including Heleomyzidae, were collected from the surface of snow in lowland and mountain areas using a semi-quantitative method. Lowland and mountain assemblages of Heleomyzidae recorded on snow were quite different. Heleomyza modesta (Meigen, 1835) and Scoliocentra (Leriola) brachypterna (Loew, 1873) dominated in the mountains, Tephrochlamys rufiventris (Meigen, 1830) mainly in the lowlands and Heteromyza rotundicornis (Zetterstedt, 1846) was common in both habitats. Heleomyzidae were found on snow during the whole period of snow cover, but the catches peaked from late November to the beginning of February. In late winter and early spring the occurrence of heleomyzids on snow decreased. Most individuals were active on snow at air temperatures between -2 and +2.5°C. A checklist of 78 winter active European Heleomyzidae is presented. Helomyza nivalis Wahlgren, 1918 is herein considered as a new junior synonym of Helomyza caesia Meigen, 1830, syn. n., Agnieszka Soszyńska-Maj, Andrzej J. Woźnica., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
18. A comparative study of Ligophorus uruguayense and L. saladensis (Monogenea: Ancyrocephalidae) from Mugil liza (Teleostei: Mugilidae) in southern Brazil
- Creator:
- Marchiori, Natalia C, Pariselle, Antoine, Pereira, Joaber, Agnèse, Jean-François, Durand, Jean-Dominique, and Vanhove, Maarten P.M
- Format:
- electronic, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- morfologie (biologie), parazitické organismy, morphology (biology), parasitic organisms, molecular systematics, mullet, taxonomy, 2, and 59
- Language:
- English
- Description:
- Representatives of Ligophorus Euzet et Suriano, 1977 were found on the gills of Mugil liza Valenciennes caught in southern Brazil. They were identified as Ligophorus uruguayense Failla Siquier et Ostrowski de Núñez, 2009 and Ligophorus saladensis Marcotegui et Martorelli, 2009, even though specific identification proved to be difficult due to inconsistencies in some diagnostic features reported for these two species. Therefore, a combined morphological and molecular approach was used to critically review the validity of these species, by means of phase contrast and confocal fluorescence microscopical examination of sclerotised hard parts, and assessing the genetic divergence between L. saladensis, L. uruguayense and their congeners using rDNA sequences. The main morphological differences between the two species relate to the shape of the accessory piece of the penis and the median process of the ventral bar. The accessory piece in L. uruguayense is shorter than in L. saladensis, has a cylindrical, convex upper lobe and straight lower lobe (vs with the distal tip of the lower lobe turning away from the upper lobe in the latter species). The ventral bar has a V-shaped anterior median part in L. uruguayense (vs U-shaped in L. saladensis). The two species are suggested to be part of a species complex together with L. mediterraneus Sarabeev, Balbuena et Euzet, 2005. We recommend to generalise such comparative assessment of species of Ligophorus for a reliable picture of the diversity and diversification mechanisms within the genus, and to make full use of its potential as an additional marker for mullet taxonomy and systematics., Natalia C. Marchiori, Antoine Pariselle, Joaber Pereira Jr., Jean-François Agnèse, Jean-Dominique Durand, Maarten P.M. Vanhove., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
19. A comparison of capture-mark-recapture and camera-based mark-resight to estimate abundance of Alpine marmot (Marmota marmota)
- Creator:
- Forti, Alessandro, Partel, Piergiovanni, Orsingher, Michel J., Volcan, Gilberto, Dorigatti, Enrico, Pedrotti, Luca, and Corlatti, Luca
- Format:
- počítač and online zdroj
- Type:
- model:article and TEXT
- Subject:
- Bowden estimator, camera traps, Marmota, and population size
- Language:
- English
- Description:
- Obtaining reliable estimates of population abundance is of utmost importance for wildlife research and management. To this aim, camera-traps are increasingly used, as this method has the advantage of being noninvasive and allows for continuous monitoring. Camera traps can be used to estimate abundance in combination with traditional capture-recapture techniques, as well as with estimators that do not require marked individuals. Here, we investigated the use of camera-based mark-recapture methods applied to an Alpine marmot (Marmota marmota) population in the Paneveggio-Pale di San Martino Natural Park (eastern Italian Alps). We compared abundance estimates derived from a traditional capture-mark-recapture (CMR) framework and camera trap mark-resight (CTMR) over three consecutive years. CMR models estimated a population size of n = 19 individuals (95% CI = 18-27), n = 15 (14-22) and n = 24 (22-32) in 2019, 2020 and 2021 respectively. CTMR returned an estimated population size of n = 24 (95% CI = 18-30), n = 20 (17-24) and n = 22 (21-24) for the same years. The difference between the estimate of these two methods was significant only in 2020, with CMR returning a lower estimate than CTMR (95% CI = –9.4-–0.6). This difference was not significant for 2019 (95% CI = –10.9-0.9) and 2021 (95% CI = –1.8-5.9). Based on our results, the use of CTMR techniques is promising in the estimation of absolute population size of marmots, and the estimator was slightly more precise than CMR. Further studies are needed to evaluate the effectiveness of CTMR with reduced capture effort.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
20. A comparison of chlorophyll fluorescence transient measurements, using Handy PEA and FluorPen fluorometers
- Creator:
- Padhi, B., Chauhan, G., Kandoi, D., Stirbet, A., Tripathy, Baishnab C., and Govindjee, G.
- Format:
- počítač and online zdroj
- Type:
- model:article and TEXT
- Subject:
- huseníček rolní, Arabidopsis thaliana, blue and red excitation light, fluorescence imaging, OJIP transient, 2, and 581
- Language:
- Multiple languages
- Description:
- We provide here a general introduction on chlorophyll (Chl) a fluorescence, then we present our measurements on fast (< 1 s) induction curves (the so-called OJIP transients) on dark-adapted intact leaves of Arabidopsis thaliana, under five different light intensities [in the range of ~ 500 to ~ 3,000 µmol(photons) m‒2 s‒1] using two different instruments: Handy PEA (Hansatech Instruments, UK; excitation light, 650 nm) and FluorPen (model FP-110; Photon Systems Instruments, The Czech Republic; excitation light, 470 nm). We then discuss the observed differences in the OJIP curves, as well as in Fo (F20μs, F50μs, or the extrapolated Ft→0), FP (the peak), and the ratios FP/Fo, and Fv (= FP ‒ Fo)/FP in terms of differences in excitation light intensity and absorptance (or absorbance) of the excitation light by the leaves, and other factors, as well as the data available in the literature. We suggest that such measurements be accompanied, in the future, by parallel measurements on Chl a fluorescence imaging, an area pioneered by Hartmut K. Lichtenthaler., B. Padhi, G. Chauhan, D. Kandoi, A. Stirbet, B. C. Tripathy, G. Govindjee., and Obsahuje bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public