A comparison of chlorophyll fluorescence transient measurements, using Handy PEA and FluorPen fluorometers

Title:

A comparison of chlorophyll fluorescence transient measurements, using Handy PEA and FluorPen fluorometers

Creator:

Padhi, B., Chauhan, G., Kandoi, D., Stirbet, A., Tripathy, Baishnab C., and Govindjee, G.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:492ea005-982b-4e3d-ad32-b4fd7acc036b
uuid:492ea005-982b-4e3d-ad32-b4fd7acc036b
issn:0300-3604
doi:10.32615/ps.2021.026

Subject:

huseníček rolní, Arabidopsis thaliana, blue and red excitation light, fluorescence imaging, OJIP transient, 2, and 581

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

We provide here a general introduction on chlorophyll (Chl) a fluorescence, then we present our measurements on fast (< 1 s) induction curves (the so-called OJIP transients) on dark-adapted intact leaves of Arabidopsis thaliana, under five different light intensities [in the range of ~ 500 to ~ 3,000 µmol(photons) m‒2 s‒1] using two different instruments: Handy PEA (Hansatech Instruments, UK; excitation light, 650 nm) and FluorPen (model FP-110; Photon Systems Instruments, The Czech Republic; excitation light, 470 nm). We then discuss the observed differences in the OJIP curves, as well as in Fo (F20μs, F50μs, or the extrapolated Ft→0), FP (the peak), and the ratios FP/Fo, and Fv (= FP ‒ Fo)/FP in terms of differences in excitation light intensity and absorptance (or absorbance) of the excitation light by the leaves, and other factors, as well as the data available in the literature. We suggest that such measurements be accompanied, in the future, by parallel measurements on Chl a fluorescence imaging, an area pioneered by Hartmut K. Lichtenthaler., B. Padhi, G. Chauhan, D. Kandoi, A. Stirbet, B. C. Tripathy, G. Govindjee., and Obsahuje bibliografické odkazy

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

399-408

Source:

Photosynthetica | 2021 Volume:59 | Number:Special issue

Harvested from:

CDK

Metadata only:

false