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Start Over Creator Ján Mojžiš

1. Anoxia / induced membrane changes in human red blood cells

Creator:
Andrej Nicák, Ján Mojžiš, Mária Jandošeková, and Ladislav Mirossay
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, cation-osmotic hemolysis, human red blood cells, anoxia, metabolic depletion, 14, and 612
Language:
English
Description:
A. Nicák, J. Mojžiš, M. Jandošeková, L. Mirossay. and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Effect of quercetin on daunorubicin-induced heart mitochondria changes in rats

Creator:
Juraj Guzy, Jaroslav Kušnír, Mária Mareková, Chavková, Z., Katarína Dubayová, Mojžišová, G., Ladislav Mirossay, and Ján Mojžiš
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, mitochondrie, mitochondrias, antioxidant enzymes, daunorubicin, quercetin, synchronous fluorescence spectra, 14, and 612
Language:
English
Description:
Cancer therapy with daunorubicin is limited by its cardiotoxicity. It has been suggested that daunorubicin-induced free radical generation can be involved. The precise molecular mechanism of daunorubicin-induced cardiotoxicity is still not well understood but it is believed that mitochondria play an important role in this process. It has been reported that flavonoids with antioxidant properties may prevent anthracycline-induced cardiotoxicity. In this work, we investigated the effects of daunorubicin and quercetin on mitochondrial enzyme activities such as ATPase, glutathione peroxidase (GPx) and glutathione reductase (GR). Moreover, we also studied the changes of outer mitochondrial membrane using synchronous fluorescence spectra. The actitivity of ATPase and GR were significantly increased after daunorubicin application. Pretreatment with quercetin significantly alleviated this increase. On the other hand, GPx activity was significantly decreased and quercetin prevented this decrease. Treatment with quercetin alone had no significant effect on the enzyme activity studied. Quercetin also completely prevented daunorubicin-induced changes in fluorescence of the outer mitochondrial membrane. In conclusion, our data indicate that quercetin may be useful in mitigating daunorubicin-induced cardiotoxicity., J. Guzy, J. Kušnír, M. Mareková, Z. Chavková, K. Dubayová, G. Mojžišová, L. Mirossay, J. Mojžiš., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. In vitro antiproliferative and antiangiogenic effects of Flavin7®

Creator:
Ján Mojžiš, Šarišský, M., Martina Pilátová, Viktória Vohárová, Varinská, L., Mojžišová, G., Alexander Ostró, Peter Urdzík, Róbert Dankovčík, and Ladislav Mirossay
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Experimentální medicína, experimentální medicína, polyfenoly, experimental medicine, polyphenols, antiproliferative, antiangiogenic, Flavin7, 14, and 616-092
Language:
English
Description:
Flavin7 (F7) is a nutritional supplement often taken by cancer patients in Central Europe during chemo- and radiation therapy. In this study, investigation of the antiproliferative and antiangiogenic activities of this supplement were performed. Flavin7 showed antiprolif erative activity in Jurkat as well as in HeLa cells. It significantly reduced the growth of both cancer cell lines at the doses of 200 μg/ml to 20 μg/ml (p<0.001 and p<0.01, respectively). In F7-treated Jurkat cells we found a significant increase in the fraction of cells with sub-G0/G1 DNA content, which is considered to be a marker of apoptotic cell death. Apoptosis was also confirmed by annexin V staining and DNA fragmentation. Furthermore, F7 at the doses of 100 μg/ml to 4 μg/ml inhibited endothelial cell migration and capillary tube formation what indicates its potential antiangiogenic properties. Flavin7 also inhibited the activity of matrix metalloproteinases (MMPs), preferentially MM P-9, at the doses of 100 μg/ml to 4 μg/ml. Our data suggest that F7 possesses marked antiproliferative and antiangiogenic properties in vitro. Further research is needed to elucidate also its in vivo activities., J. Mojžiš, M. Šarišský, M. Pilátová, V. Voharová, L. Varinská, G. Mojžišová, A. Ostro, P. Urdzík, R. Dankovčik, L. Mirossay., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Modulation of the Phototoxic Effect of Hypericin in Human Leukemia CEM Cell Line by N-Ethylmaleimide, Amiloride and Omeprazole

Creator:
Andrej Miroššay, Ladislav Mirossay, Šarišský, M., Papp, P., and Ján Mojžiš
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, Hypericin, Phototoxicity, N-ethylmaleimide, Amiloride, Omeprazole, CEM cells, 14, and 612
Language:
English
Description:
Hypericin is a photosensitizing plant pigment from Hypericum perforatum with multiple modes of light-induced biological activities due to production of singlet oxygen and/or excited-state proton transfer with consequent pH drop in the hypericin environment. In the present work, we studied the effects of three inhibitors of crucial mechanisms responsible for intracellular pH (pHi) regulation on hypericin phototoxicity: N-ethylmaleimide (NEM), an inhibitor of H+-ATPase, 5'-(N,N-dimethyl)-amiloride (DMA), an inhibitor of Na+/H+ exchanger, and omeprazole (OME), an inhibitor of H+K+-ATPase. Our experiments show that the effect of hypericin at 1x10-5 and 1x10-6 mol.l-1 was significantly potentiated by NEM (1x10-7-1x10-9 mol.l-1) and DMA (1x10-6 and 1x10-7 mol.l-1) in leukemic CEM cell line. On the other hand, OME had no significant effect on hypericin cytotoxicity. Our results support the hypothesis that the excited-state proton transfer and the consequent acidification of hypericin environment could play a role in the biological activity of hypericin., A. Miroššay, L. Mirossay, M. Šarišský, P. Papp, J. Mojžiš., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

5. Role of mucus in ischemia/reperfusion-induced gastric mucosal injury in rats

Creator:
Ján Mojžiš, Renáta Hegedüšová, and Ladislav Mirossay
Format:
print, bez média, and svazek
Type:
article, články, journal articles, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, ischemie, ischemia, ischemia/reperfusion, gastric mucus, sucralfate, malotilate, n-acetylcysteine, 14, and 612
Language:
English
Description:
Gastric mucus plays an important role in gastric mucosal protection. Apart from its “barrier” function, it has been demonstrated that mucus protects gastric epithelial cells against toxic oxygen metabolites derived from the xanthine/xanthine oxidase system. In this study, we investigated the effect of malotilate and sucralfate (mucus production stimulators) and N-acetylcysteine (mucolytic agent) on ischemia/reperfusion-induced gastric mucosal injury. Gastric ischemia was induced by 30 min clamping of the coeliac artery followed by 30 min of reperfusion. The mucus content was determined by the Alcian blue method. Sucralfate (100 mg/kg), malotilate (100 mg/kg), and N-acetylcysteine (100 mg/kg) were given orally 30 min before surgery. Both sucralfate and malotilate increased the mucus production in control rats. On the other hand, N-acetylcysteine significantly decreased mucus content in control (sham) group. A significant decrease of mucus content was found in the control and the N-acetylcysteine pretreated group during the period of ischemia. On the other hand, sucralfate and malotilate prevented the decrease the content of mucus during ischemia. A similar result can be seen after ischemia/reperfusion. In the control group and N-acetylcysteine pretreated group a significant decrease of adherent mucus content was found. However, sucralfate and malotilate increased mucus production (sucralfate significantly). Sucralfate and malotilate also significantly protected the gastric mucosa against ischemia/reperfusion-induced injury. However, N-acetylcysteine significantly increased gastric mucosal injury after ischemia/reperfusion. These results suggest that gastric mucus may be involved in the protection of gastric mucosa after ischemia/reperfusion., J. Mojžiš, R. Hegedüšová, L. Mirossay., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

6. The effect of quercetin on light-induced cytotoxicity of hypericin

Creator:
Andrej Miroššay, Onderková, H., Ladislav Mirossay, Šarišský, M., and Ján Mojžiš
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, hypericin, quercetin, cell line, oxygen radicals, cytotoxic activity, 14, and 612
Language:
English
Description:
Protective effect of quercetin, a natural antioxidant compound, on hypericin-induced cytotoxicity was studied in human promyelocytic leukemia cells (HL-60). Hypericin (10-5 mol.l-1) alone significantly decreased cell survival to 21 % that found in the controls, whereas in combination with quercetin (10-5 mol.l-1) this decrease was diminished to 46 %. Lower concentrations of quercetin had no protective effect. These findings indicate that oxygen radicals can play an important role in hypericin-induced phototoxic effects., A. Miroššay, H. Onderková, L. Mirossay, M. Šarišský, J. Mojžiš., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public