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1. Hypericin - Inducted Phototoxicity of Human Leukemic Cell Line HL - 60 is Potentiated by Omeprazole, an Inhibitor of H+K+ - ATP and 5' - (N,N-dimethyl) - amiloride, an Inhibitor of Na+/H+ Exchanger

Creator:
Mirossay, L., Miroššay, A., Kočišová, E., Radváková, I., Miškovský, P., and Mojžiš, J.
Type:
article, model:article, and TEXT
Subject:
Hypericin, Omeprazole, Amiloride, HL-60, and Cytotoxic activity
Language:
English
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Modulation of the Phototoxic Effect of Hypericin in Human Leukemia CEM Cell Line by N-Ethylmaleimide, Amiloride and Omeprazole

Creator:
Andrej Miroššay, Ladislav Mirossay, Šarišský, M., Papp, P., and Ján Mojžiš
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, Hypericin, Phototoxicity, N-ethylmaleimide, Amiloride, Omeprazole, CEM cells, 14, and 612
Language:
English
Description:
Hypericin is a photosensitizing plant pigment from Hypericum perforatum with multiple modes of light-induced biological activities due to production of singlet oxygen and/or excited-state proton transfer with consequent pH drop in the hypericin environment. In the present work, we studied the effects of three inhibitors of crucial mechanisms responsible for intracellular pH (pHi) regulation on hypericin phototoxicity: N-ethylmaleimide (NEM), an inhibitor of H+-ATPase, 5'-(N,N-dimethyl)-amiloride (DMA), an inhibitor of Na+/H+ exchanger, and omeprazole (OME), an inhibitor of H+K+-ATPase. Our experiments show that the effect of hypericin at 1x10-5 and 1x10-6 mol.l-1 was significantly potentiated by NEM (1x10-7-1x10-9 mol.l-1) and DMA (1x10-6 and 1x10-7 mol.l-1) in leukemic CEM cell line. On the other hand, OME had no significant effect on hypericin cytotoxicity. Our results support the hypothesis that the excited-state proton transfer and the consequent acidification of hypericin environment could play a role in the biological activity of hypericin., A. Miroššay, L. Mirossay, M. Šarišský, P. Papp, J. Mojžiš., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public