Biometric data are typically used for the purposes of unique identification of a person. However, recent research suggests that biometric data gathered for the purpose of identification can be analysed for extraction of additional information. This augments indicative value of biometric data. This paper illustrates the range of augmented indicative values of the data as well as identifies crucial factors that contribute to increased vulnerability of data subjects., Alžběta Krausová, Hananel Hazan, Ján Matejka., and Obsahuje bibliografické odkazy
The retina is characterized by glycolysis under aerobic conditions, mediated by lactate dehydrogenase isoenzyme-5 (LDH-5) as well as by the soluble isoenzyme of malate dehydrogenase. Bovine retina LDH and MDH isoenzymes and their activities were studied after polyamine treatment. Our results showed that LDH-5 isoenzyme presented the highest activity in untreated as well as in putrescine-treated retina. Decreased activity was present when the retina was treated with spermidine or spermine. It was demonstrated that retinic LDH-5 had a high affinity for lactate which enabled the isoenzyme to be more effective than the other LDH isoenzymes in the conversion of NADH to NAD. Therefore, the putrescine enhancing LDH-5 activity appeared to be capable of stimulating NAD-mediated rhodopsin regeneration. Putrescine induced a marked increase of both MDH isoenzymes - soluble (s-MDH) and mitochondrial (m-MDH), while spermine and spermidine mostly affected the soluble form of the enzyme. Putrescine induced a three-fold increase in s-MDH and m-MDH activities, while spermine and spermidine induced a four to five-fold increase in s-MDH. These results document the differential effects of polyamine treatment on LDH and MDH isoenzyme activities., I. Venza, A. Valenti, P. Ruggeri, L. Denaro, D. Teti., and Obsahuje bibliografii
''In the middle of 1964 brought Dr. John to our laboratory a girl with quite torn eye retina. Neither classical surgery operation nor the Lichtkoagulator device was applicable. She had the only chance and that was the application of laser radiation ... '' remember nestors of Czech laser physics and quantum electronics Jan Blabla and Viktor Trkal. and ''V polovině roku 1964 přivedl MUDr. John do naší laboratoře děvče, které mělo zcela roztrhanou sítnici. Pro lékařský zákrok nebylo možné použít klasický postup operace, ani operaci pomocí nekoherentního Lichtkoagulátoru. Pacientka měla jedinou šanci, a tou byla aplikace laserového záření ...'' vzpomínají nestoři české laserové fyziky a kvantové elektroniky Jan Blabla a Viktor Trkal.
Cytochrome oxidase activity from the retina can be enhanced or depressed by free radical-mediated reactions both in positive and negative aspect. The greatest effect was exerted by ischemia/reperfusion, which significantly increased the fluorescent products of lipid peroxidation (358 %, P<0.01) and inhibited the enzyme activity (14 %, P<0.001). After hyperoxia the fluorescent products slightly increased (192 %, P< 0.05) as well as the enzyme activity (133 %, P<0.05). Hypoxia had no effect on any of these parameters. Specific changes in the composition of fluorophores after ischemia/reperfusion were revealed in the fluorescence spectra. The fact that increased lipid peroxidation after hyperoxia and after ischemia/reperfusion does not produce the same effect upon cytochrome oxidase activity might be explained by changes in the kinetic behavior of cytochrome oxidase. In the control enzyme preparation, two binding sites for cytochrome c were observed. One was of the low-affinity (Km=60 mM) and the other of the high-affinity (Km=1.12 mM). After in vitro-initiated lipid peroxidation, the low-affinity binding site was lost and the activity measured under "optimum" conditions at a single cytochrome concentration was higher than in the controls. This implies that oxidative damage to cytochrome oxidase in vivo can be site-specific and its extent should be estimated by performing detailed kinetic analysis as otherwise the results might be misleading., A. Šišková, J. Wilhelm., and Obsahuje bibliografii