The regeneration of the sciatic nerve after microsuture was compared with the connection of transected nerve with a coagulum of autologous blood plasma in 20 rabbits. The epineuroperineural suture was performed in 10 rabbits (group A). The severed nerve was approximated with fibrin glue of autologous blood plasma in 10 rabbits (group B). Their skin sensation margin during a 3-month-period of regeneration was examined, 90 days after surgery the connection was inspected and the nerve conduction velocity was measured across the site of the anastomosis. The microsuture was found to be firm in all 10 animals of group A. On the other hand, in 2 animals of group B, the glue failed to keep the nerve stumps approximated (dehiscence occurred in 20 % of the animals). There were no significant differences found on clinical and electrophysiological testing of regenerated nerves of both groups. The method of autologous fibrin glue in the repair of peripheral nerve transection does not provide a sufficiently firm connection. This procedure with the preparation of the centrifuged plasma is a more time-consuming method in comparison with the microsuture. Epineuroperineural microsuture with maximal effort to adapt the corresponding nerve fibres remains the method of choice for peripheral nerve reconstruction.
Various stages in the succession of vegetation of peat bogs following disturbance were studied in the Třeboň Basin, Czech Republic. The disturbance was of two types: (a) natural, represented by windthrow, with subsequent bark beetle attack, and fire, and (b) human-made peat digging and industrial peat milling. The species composition at different stages in succession following disturbance were compared with that in undisturbed plots. Regeneration of peat bog vegetation was faster after a natural than after human-made disturbance. The lowest impact was caused by windthrow, followed by fire. Regeneration after peat digging took much longer. Regeneration after industrial peat harvesting only occurred if the groundwater table level remained high.
The objective of the current study is to present data on the
splitting of skeletal muscle fibers in C57BL/6NCrl mice. Skeletal
muscles (m. rectus femoris (m. quadriceps femoris)) from 500
(250 ♀ and 250 ♂) C57BL/6NCrl mice in the 16th week of life
were sampled during autopsy and afterwards standardly
histologically processed. Results show spontaneous skeletal
muscle fiber splitting which is followed by skeletal muscle fiber
regeneration. One solitary skeletal muscle fiber is split, or is in
contact with few localized splitting skeletal muscle fibers. Part of
the split skeletal muscular fiber is phagocytosed, but the
remaining skeletal muscular fiber splits are merged into one
regenerating skeletal muscle fiber. Nuclei move from the
periphery to the regenerating skeletal muscle fiber center during
this process. No differences were observed between female and
male mice and the morphometry results document <1 % skeletal
muscle fiber splitting. If skeletal muscular fibers splitting occurs
5 %> of all skeletal muscular fibers, it is suggested to describe
and calculate this in the final histopathological report.
We propose a modified and updated protocol to obtain mitotic chromosomes from the regenerated tissue of Pelophylax tadpole tail tips. Chromosomal preparations from regenerated tissue results in high-quality and clean slides suitable for further staining and study. Tadpoles remain alive, undergo minimum suffering, and can be grown to adulthood for further investigation. The method could be used for other groups of Anura and modified for other species with the ability to regenerate their tissues.
Mitochondria were isolated from regenerating rat liver 12, 24 and 48 h after partial hepatectomy. The "State 3" and "State 4" respiration were measured in the presence of succinate. The P/O quotient and respiratory control index (RCI) were calculated. The experimental data showed that the partial uncoupling of oxidative phosphorylation in regenerating liver mitochondria occurring in the early period of regeneration is partly due to free fatty acids.
In the present study we investigated the effect of a two-stage bilateral lesion of the olfactory bulb (OB) in rats on the regeneration ability of peripheral olfactory neurons and their reinnervation capacity in the spared OB. The outgrowth of newly-generated olfactory axons as well as the maturation of their terminal synaptic field was detected by immunohistochemistry of the growth-associated phosphoprotein B-50/GAP-43. In addition, the glial response to the surgery was monitored by an immunohistochemical marker for astrocytes, glial fibrillary acidic protein (GFAP). In neonatal rats (P3-P5), the right OB was removed, then three months later the contralateral side was ablated. Six days after the second operation the animals were transcardially perfused. Their brains were embedded in paraplast, serially sectioned and processed for histological and immunohistochemical observations. After neonatal OB ablation, homogeneous B-50-immunoreactivity (BIR) was found in the forebrain, olfactory axons and ectopic glomeruli localized in the small OB remnant-like structures and in the regenerated neuroepithelium. A strong GFAP response was revealed in the brain cortex as well as in the newly-formed olfactory axons and glomeruli-like structures of the OB remnants. After adult OB ablation strong BIR was observed in olfactory axons, while remaining glomerular structures were only faintly stained. The neuroepithelium revealed signs of massive degenerative processes with a substantial decrease in BIR. The GFAP-positive astrocytes were scattered throughout the entire OB remnant and were prominent in the glomeruli-like structures and adjacent frontal cortex. In the present study, we applied GAP-43 and GFAP immunohistochemistry to characterize the responses of individual olfactory components after two-stage olfactory bulbectomy. Furthermore, this model of OB ablation characterized by two immunohistochemical markers could elucidate certain molecular mechanisms involved in the regeneration and/or plasticity of the olfactory system.