The objective of the present experiment was to assess the involvement of small intestine in expression of susceptibility or resistance to the high-fat/high-energy diet. The investigation was carried out in adult male Sprague-Dawley rats fed either standard laboratory diet (3.2 kcal/g, 9.5 % fat) or high-fat (HF) diet (4.04 kcal/g, 30 % fat) for 4 weeks as well as in HF rats that were retrospectively designated on the bases of their higher or lower weight gain as sensitive (DIO) or resistant (DR) to obesity. Our results revealed in HF group significant increase in energy intake, food efficiency, weight gain and Lee´s index of obesity. Moreover, in comparison with controls, a significantly increased duodenal and jejunal alkaline phosphatase (AP) and α-glucosidase activity as well as hypertrophy of jejunal mucosa (increased protein/DNA ratio) were observed in HF fed rats. In contrast, intestinal function was inversely related to energy intake or to the development of adiposity in DIO vs. DR rats. The DR rats had significantly greater AP and α-glucosidase activity and more pronounced suppression of energy intake than obese DIO rats. It indicates that the increase of enzyme activities and the lowered effectiveness of nutrient absorption might be a significant factor preventing the expression of obesity proneness. This information contributes to a better understanding of a complex interaction between HF diet feeding and small intestinal adaptability, which determines the energy homeostasis and predict the ability to resist or develop obesity in these phenotypes., Z. Šefčíková, T. Hájek, Ľ. Lenhardt, Ľ. Raček, Š. Možeš., and Obsahuje bibliografii a bibliografické odkazy
Dr. techn. PhDr. Jaroslav Just a Ing. Vojtěch Straka, Nad názvem: Zemědělská rada pro království České. Český odbor, Číslo tisku CCLII. Číslo jednací 21512/1917, and Converted from MODS to DC version 1.8 (EE patch 2018/05/24)
spolupracovníci: V. Weger, J. Jeřábek, J. Tupý, J. Mráz, F. Skořepa, E. Sova, J. Tejčka, Fr. Živnůstka and Converted from MODS to DC version 1.8 (EE patch 2018/05/24)
dle mnoholetých zkušeností sestavil a vzhledem na nynější hospodaření sepsal Karel Lambl and Converted from MODS to DC version 1.8 (EE patch 2018/05/24)