The general population is potentially exposed to many chemicals that can affect the endocrine system. These substances are called endocrine disruptors (EDs), and among them bisphenol A (BPA) is one of the most widely used and well studied. Nonetheless, there are still no data on simultaneous measurements of various EDs along with steroids directly in the seminal fluid, where deleterious effects of EDs on spermatogenesis and steroidogenesis are assumed. We determined levels of BPA and 3 estrogens using LC-MS/MS in the plasma and seminal plasma of 174 men with different degrees of infertility. These men were divided according their spermiogram values into 4 groups: (1) healthy men, and (2) slightly, (3) moderate, and (4) severely infertile men. Estradiol levels differed across the groups and body fluids. Slightly infertile men have significantly higher BPA plasma and seminal plasma levels in comparison with healthy men (p<0.05 and p<0.01, respectively). Furthermore, seminal BPA, but not plasma BPA, was negatively associated with sperm concentration and total sperm count (-0.27; p<0.001 and -0.24; p<0.01, respectively). These findings point to the importance of seminal plasma in BPA research. Overall, a disruption of estrogen metabolism was observed together with a weak but significant impact of BPA on sperm count and concentration., J. Vitku, L. Sosvorova, T. Chlupacova, R. Hampl, M. Hill, V. Sobotka, J. Heracek, M. Bicikova, L. Starka., and Obsahuje bibliografii
Polycystic ovary syndrome (PCOS) is commonly associated with
a higher cardiometabolic risk. The relationship between steroid
hormones and cardiometabolic profile in PCOS has been
evaluated, but no single hormonal predictor of this association
has been identified to determine. To determine the relationship
between steroid hormones and cardiometabolic risk factors in
PCOS women. Study included 64 women diagnosed with PCOS.
Fasting blood samples were analyzed for biochemical, metabolic
parameters and sex steroid hormones. PCOS women with
BMI≥27 had significantly higher serum free testosterone (FT),
free androgen index (FAI), estrone (E1) (p=0.014, p=0.02,
p=0.01) than those with normal weight. In all subjects
E1 positively correlated with BMI (p=0.0067), serum insulin
(p=0.0046), HOMA-IR (p=0.0125) and negatively with
HDL-cholesterol (p=0.009). FAI positively correlated with serum
cholesterol (p=0.0457), triacylglycerols (TAG) (p=0.0001),
HOMA-IR (p=0.037), and glycemia (p=0.0001), negatively with
HDL-cholesterol (p=0.029). In multiple linear regression model
E1 most significantly predicted HOMA-IR, whereas FT/FAI
predicted HDL-cholesterol and BMI. We conclude that PCOS
women with marked overweight or obesity have higher FT, FAI
and E1 as compared with nonobese PCOS subjects. E1 and FT
may predict worse cardiometabolic profile in PCOS.