The general population is potentially exposed to many chemicals that can affect the endocrine system. These substances are called endocrine disruptors (EDs), and among them bisphenol A (BPA) is one of the most widely used and well studied. Nonetheless, there are still no data on simultaneous measurements of various EDs along with steroids directly in the seminal fluid, where deleterious effects of EDs on spermatogenesis and steroidogenesis are assumed. We determined levels of BPA and 3 estrogens using LC-MS/MS in the plasma and seminal plasma of 174 men with different degrees of infertility. These men were divided according their spermiogram values into 4 groups: (1) healthy men, and (2) slightly, (3) moderate, and (4) severely infertile men. Estradiol levels differed across the groups and body fluids. Slightly infertile men have significantly higher BPA plasma and seminal plasma levels in comparison with healthy men (p<0.05 and p<0.01, respectively). Furthermore, seminal BPA, but not plasma BPA, was negatively associated with sperm concentration and total sperm count (-0.27; p<0.001 and -0.24; p<0.01, respectively). These findings point to the importance of seminal plasma in BPA research. Overall, a disruption of estrogen metabolism was observed together with a weak but significant impact of BPA on sperm count and concentration., J. Vitku, L. Sosvorova, T. Chlupacova, R. Hampl, M. Hill, V. Sobotka, J. Heracek, M. Bicikova, L. Starka., and Obsahuje bibliografii
Natural and synthetic compounds called endocrine-disrupting chemicals can interfere with normal hormone binding to convey inaccurate signals or send mixed messages that may result in altered health outcomes of both wildlife and humans. Estriol is one of natural origin endocrine disruptors and it is metabolite of 17β-estradiol. The aim of this work was to develop a method for determining free estriol available to capaciting sperm. In order to determine a status of estriol during mouse sperm capacitation in vitro a high performance liquid chromatography HPLC method with UV detection was used. A free estriol, and the estriol bound to the bovine serum albumin in capacitation medium can be quantified by the proposed method. A reversed-phase separation mode using a SunFire C18 column with a simple mobile phase composed of acetonitrile and water, methanol and water at the ratio 40/60 (v/v) was applied. Our results show that the level of free estriol available for mouse spermatozoa during capacitation in vitro can be quantified by HPLC method with UV detection. Therefore, this method represents an important tool to determine the amount of environmental estrogens, such as estriol, bound to sperm cells at the specific time point of capacitation in vitro.