Representatives of the Academy of Science of Czech Republic and Charles University have subscribed to the Founding Contract of the Biotechnological and Biomedical Center (BIOCEV) that will be situated in the vicinity of Vestec. and Luděk Svoboda.
The Academy of Sciences of the Czech Republic (AS CR) established a new public research institution, the Institute of Biotechnology AS CR, v. v. i. The primary ambition of this new institute is to develop cutting-edge basic and oriented research on topics opening for diagnostic and therapeutic applicatons in human medicine. In particular, the institute was established to serve as a nucleation center of BIOCEV, the joined Biotech & Biomed Research Center of the Academy of Sciences with Charles University, to be built at Vestec near Prague by the year 2012 with the support of the European Regional Development Funds in frame of the Operational Program R&D for Innovation., Peter Šebo, -red-., and Tři otázky pro ředitele připravila -red-
The research activities of this Centre are focused on the study of cyanobacteria, algae and symbiotic soil microorganisms (bacteria and algae), which are used for bioindicatoin and revitalization of toxic anthropogenic substrates and water resources. The targeted outcomes are methods for predicting cyanobacteria in drinking wter and water resources, technology of biogas production from algal biomass and the use of algal biomass for the pharmaceutical industry and biotechnology of microbial inoculations for more effective recultivation of brown-coal spoil banks. The research Centre is divided into three research groups each dealing with a portion of the research task: Group 1 - Cyanobacteria and their toxins in the waterworks industry; Group 2 - Use of algae for bioindication and revitalization of polluted water and biomass use; Group 3 - Use of microbial inoculations for soil revitalization. and Miroslav Vosátka, Jana Albrechtová.
In the present in vitro experiments we examined FSH- and ghrelin-induced changes in ovarian hormone secretion by transgenic rabbits. Fragments of ovaries isolated from adult transgenic (carrying mammary gland-specific mWAP-hFVIII gene) and non-transgenic rabbits from the same litter were cultured with and without FSH or ghrelin (both at 0, 1, 10 or 100 ng/ml medium). The secretion of progesterone (P4), estradiol (E2) and insulin-like growth factor I (IGF-I) was assessed by RIA. It was observed that ovaries isolated from transgenic rabbits secreted much less P4, E2 and IGF-I than the ovaries of non-transgenic animals. In control animals FSH reduced E2 (at doses 1-100 ng/ml medium) and IGF-I (at 1-100 ng/ml), but not P4 secretion, whereas ghrelin promoted P4 (at 1 ng/ml) and IGF-I (at 100 ng/ml), but not E2 output. In transgenic animals, the effects were reversed: FSH had a stimulatory effect on E2 (at 100 ng/ml) and ghrelin had an inhibitory effect on P4 (at 10 ng/ml). No differences in the pattern of influence of FSH on P4 and IGF-I and of ghrelin on E2 and IGF-I were found between control and transgenic animals. The present observations suggest that 1) both FSH and ghrelin are involved in rabbit ovarian hormone secretion, 2) transgenesis in rabbits is associated with a reduction in ovarian secretory activity, and 3) transgenesis can affect the response of ovarian cells to hormonal regulators., A. V. Sirotkin, P. Chrenek, K. Darlak, F. Valenzuela, Ž. Kuklová., and Obsahuje bibliografii a bibliografické odkazy