In the European pine vole (Microtus subterraneus, Arvicolidae, Rodentia) eight palatal rugae are formed, three of them are located in the antemolar, five in the intermolar area. The development of these rugae was studied in staged and aged embryos and fetuses using light and scanning electron microscopy. In 12.5 to 13.5 DO (days of ontogeny) embryos, epithelial thickening of ruga 1 was observed, whilst rugae 2 to 4 appeared as epithelial buds in rostral parts of the palatal processes; in their caudal parts, epithelial thickening was discernible (future rugae 5 to 8). The cell surface was characterised by rarely dispersed minute cytoplasmatic projections, sometimes arranged into rows along cell borders. In embryos at 14 to 16 DO, the secondary palate was closed and eight rugae were observed as in newborn voles. Rugae 1 to 3 distinctly protruded into the oral cavity. In fetuses at 17 to 18 DO, formation of the mesenchymal core in palatal rugae occured in rostro-caudal direction. SEM revealed simple-shaped microvilli and microplicae on the epithelial surfaces. In fetuses at 18 to 18.5 DO, the shape of the epithelial base was changed beneath ruga 2 to 6. The rugal core was distinctly developed in all rugae. The microplicae were thicker, acquired more complex shape and became interlaced. In fetuses at 19 to 19.5 DO, the special formation of the epithelial base beneath ruga 2 to 6 continued. The cell surface was covered by a dense network of microplicae the mass of which considerably increased.
The development of the nematode Procamallanus saccobranchi Karve, 1952, a parasite in the stomach of the fish Heteropneustes fossilis (Bloch), was studied in Mesocyclops crassus (Fischer) and Mesocyclops leuckarti (Claus). After being ingested by the copepods the nematode first-stage larvae penetrated into the haemocoel of the intermediate host; there they moulted twice (on days 3 and 5 p.i. at 28-30°C) attaining the third, infective stage. The definitive host H. fossilis acquired infection by feeding on copepods harbouring infcclivc-stage larvae; in the stomach of this definitive host, the larvae were observed to undergo two more moults. The third moult occurred on day 13 p.i. and the fourth moult on day 38 p.i. and day 66 p.i. in “male” and “female” larvae, respectively. The larval stages, including the moulting forms are described and illustrated.
b1_We newly elaborated and adapte d several radiometric enzyme assays for the determination of activities of the key enzymes engaged in the biosynthesis (thyroid peroxidase, TPO) and metabolic transformations (conjugating enzymes and iodothyronine deiodinases, IDs) of thyroid hormones (THs) in the thyroid gland and in peripheral tissues, especially in white adipose tissue (WAT). We also elaborated novel, reliable radiometric methods for extremel y sensitive determination of enzyme activities of IDs of types 1, 2 and 3 in microsomal fractions of different rat and hum an tissues, as well as in homogenates of cultured mammalia n cells. The use of optimized TLC separation of radioactive products from the unconsumed substrates and film-less autoradiography of radiochromatograms, taking advantage of storage phosphor screens, enabled us to determine IDs enzyme activities as low as 10-18 katals. In studies of the interaction of fluoxetine (Fluox) with the metabolism of THs, we applied adapted radiometric enzyme assays for iodothyronine sulfotransferases (ST) and uridine 5’-diphosphoglucuronyltransferase (UDP-GT). Fluox is the most frequently used representative of a new group of non-tricyclic antidepressant drugs - selective serotonin re-uptake inhibitors. We used the elaborated assays fo r quantification the effects of Fluox and for the assessment of th e degree of potential induction of rat liver ST and/or UDP-GT enzyme activities by Fluox alone or in combination with T3 . Furthermore, we studied possible changes in IDs activities in murine adipose tissue under the conditions that promoted either tissue hypertrophy (obesogenic treatment) or involution (caloric restriction), and in response to leptin, using our newly developed radiometric enzyme assays for IDs., b2_Our results suggest that deiodinase D1 has a functional role in WAT, with D1 possibly being involved in the control of adipose tissue metabolism and/or accumulation of the tissue. Significant positive correlation between specific enzyme activity of D1 in WAT and plasma leptin levels was found. The newly developed and adapted radiometric enzyme assays proved to be very useful tools for studies of factors modulating THs metabolism, not only in model animals but also in clinical studies of human obesity., S. Pavelka., and Obsahuje bibliografii a bibliografické odkazy
This study presents data about the effect of parent material on the intensity of processes that lead to the formation of a cambic subsurface horizon. The study was performed in the Voděradské bučiny National Nature Reserve with granite bedrock and in Humpolec with paragneiss bedrock. Representative soil profiles in the southeastern part of Bohemia were characterised on a macroscale level based on macromorphological description, particle size distribution, chemical, physical and soil organic matter properties. On the basis of the values of organic carbon and bulk density, the stock of soil organic matter was calculated in the upper 25 cm of soils. A more detailed characteristic of soil cover employed micromorphological and X-ray diffraction analyses. The results revealed differences in the formation of the cambic horizon on different types of parent material. The main soil forming process responsible for the cambic horizon is more intensive at localities with paragneiss bedrock., Anna Žigová, Martin Šťastný and Radka Kodešová., and Obsahuje bibliografii
The development of the nematode Spinitectus inermis (Zeder, 1800), a parasite of the stomach of eels, Anguilla anguilla (L.) in Europe, was experimentally studied. Mayfly nymphs Caenis macrura, Ecdyonurus dispar, Heptagenia sulphurea, Potamanthus luteus and Seratella ignita from Portugal and the Czech Republic were found to serve as experimental intermediate hosts. After ingestion of the nematode eggs by the mayfly nymphs, the toothed first-stage larvae were released and penetrated into the body cavity of the intermediate host. There they moulted twice (on day 4 and 6 post infection [p.i.] at water temperatures of 20-25°C), attaining the third infective stage. The definitive host, A. anguilla, undoubtedly acquires infection by feeding on mayfly nymphs harbouring infective-stage larvae. In an experimentally infected eel, the fourth-stage larva undergoing the third moult was observed 28 days p.i. at water temperature of 20ºC. The larval stages, including moulting forms, are described and illustrated. The prepatent period of S. inermis is estimated to be about two months.
Fifty-day-old fry of tilapia hybrids (Oreochromis aureus x niloticus) were placed in aquaria containing sediment with oocysts of Eimeria (sensu lato) vanasi Landsberg et Paperna. In the first 29 h after exposure sporulated oocysts in the stomach and free sporozoites in the gut could be found in examined fish. By 7 to 56 h after exposure, sporozoites, with their characteristic crystalloid body, were detected in intraepithélial lymphocyte-like and other leucocyte-like cells, but never in the epithelial cells. Infected cells were confined to the epithelial layer and did not enter the lamina propria. Within this time, some of the sporo-zoitcs divided by endodyogeny, once or twice in succession, to form daughter sporozoites. The parent’s sporozoite crystalline body was divided between the offspring of the primary and secondary divisions.
This paper deals with the basic dynamical model of electromotor whose creation motivated by problems of electromotor vibrations and its subsequent fatigue. The model is chaacrterized by a flexible shaft with mounted padket of sheet metals that are equipped with parallel copper bars connected by end shortcircuit rings. Finite element analysis is used for the disretization of the shaft, while the sheet metal packet of cylindrical shape is modelled as a set of rigid bodies joined using chosen viscoelastic forces and torques. The shortcircuit rings are supposed to be rigid in this basic dynamical model and the pieces of copper bars are substituted by massless springs of calculated properties. Problematic model parameters are identified by means of performed experimental modal analysis. and Obsahuje seznam literatury
AIM: The purpose of this study was to develop a revised version of the Brief Bedside Dysphagia Screening Test for determining penetration/aspiration risk in patients prone to dysphagia. The priority was to achieve high sensitivity and negative predictive value. METHODS: The study screeners conducted bedside assessment of the swallowing function in 157 patients with a neurological (mainly stroke) or an ear, nose, and throat diagnosis (mainly head and neck cancer). The results were compared with a gold standard, flexible endoscopic examination of swallowing. RESULTS: For the neurological subgroup (N = 106), eight statistically significant bedside assessment items were combined into the Brief Bedside Dysphagia Screening Test-Revised (BBDST-R). Cut-off score 1 produced the highest sensitivity (95.5%; 95% confidence interval CI [CI]: 84.9-98.7%) and negative predictive value (88.9%; 95% CI 67.2-96.9%). CONCLUSION: The BBDST-R is suitable for dysphagia screening in departments caring for patients with neurological conditions. and P. Mandysová, E. Ehler, J. Škvrňáková, M. Černý, I. Bártová, A. Pellant