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Start Over Creator Gavrilović, L.

1. Changes of hippocampal noradrenergic capacity in stress condition

Creator:
Gavrilović, L., Popović, N., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I., and Pajović, S. B.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
chronic restraint stress, noradrenaline, hippocampus, and rats
Language:
English
Description:
This study aimed to investigate the effects of chronic restraint stress (CRS) on the protein levels of dopamine-β-hydroxylase (DBH), noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and brain-derived neurotrophic factor (BDNF), as well as the concentration of noradrenaline (NA) in the rat hippocampus. The investigated parameters were quantified by Western blot analyses and ELISA kits. We found that CRS increased the protein levels of DBH by 30 %, VMAT2 by 11 %, BDNF by 11 % and the concentration of NA by 104 %, but decreased the protein levels of NET by 16 % in the hippocampus of chronically stressed rats. The molecular mechanisms by which CRS increased the hippocampal NA level are an important adaptive phenomenon of the noradrenergic system in the stress condition.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

2. Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models

Creator:
Filipović, D., Gavrilović, L., Dronjak, S., Demajo, M., and Marija B. Radojčić
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Biochemie. Molekulární biologie. Biofyzika, molekulární biologie, potkan, játra, stres (fyziologie), glukokortikoidy, molecular biology, Rattus norvegicus, liver, stress (physiology), glucocorticoids, protein 70 teplotního šoku, heat shock protein 70, 2, and 577
Language:
English
Description:
The aim of the present study was to define the stress-induced pattern of cytosolic glucocorticoid receptor (GR) and Hsp70 protein in the liver of male Wistar rats exposed to different stress models: acute (2 h/day) immobilization or cold (4 °C); chronic (21 days) isolation, crowding, swimming or isolation plus swimming and combined (chronic plus acute stress). Changes in plasma levels of corticosterone were studied by radioimmunoassay (RIA). The results obtained by Western immunoblotting showed that both acute stressors led to a significant decrease in cytosolic GR and Hsp70 levels. Compared to acute stress effects, only a weak decrease in the levels of GR and Hsp70 was demonstrated in chronic stress models. Chronically stressed rats, which were subsequently exposed to novel acute stressors (immobilization or cold), showed a lower extent of GR down-regulation when compared to acute stress. The exception was swimming, which partially restores this down-regulation. The observed changes in the levels of these major stress-related cellular proteins in liver cytosol lead to the conclusion that chronic stressors compromise intracellular GR down-regulation in the liver., D. Filipović, L. Gavrilović, S. Dronjak, M. Demajo, M. B. Radojčić., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public