Red region excitation spectra of protochlorophyllide in dark-grown leaves from plant species with different proportions of its spectral forms

Title:

Red region excitation spectra of protochlorophyllide in dark-grown leaves from plant species with different proportions of its spectral forms

Creator:

Amirjani, M. R. and Sundqvist, C.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:e35b80f2-ea04-4f96-bf9d-cc2d33a52501
uuid:e35b80f2-ea04-4f96-bf9d-cc2d33a52501
issn:0300-3604
doi:10.1007/s11099-005-0162-3

Subject:

chlorophyllide, etioplast, fluorescence spectrum, mutants, Pisum, species differences, Triticum, and Zea

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Etiolated leaves of three different species, maize, wheat, and pea, as well as a pea mutant (lip1) were used to compare the excitation spectra of protochlorophyllide (Pchlide) in the red region. The species used have different composition of short-wavelength and long-wavelength Pchlide forms. The relation between different forms was furthermore changed through incubating the leaves in 5-aminolevulinic acid (ALA), which caused an accumulation of short-wavelength Pchlide forms, as shown by changes in absorption and fluorescence spectra. This is the first time a comprehensive comparison is made between excitation spectra from different species covering an emission wavelength range of 675-750 nm using fluorescence equipment with electronic compensation for the variations in excitation irradiance. The different forms of Pchlide having excitations peaks at 628, 632, 637, 650, and 672 nm could be best measured at 675, 700, 710, 725, and 750 nm, respectively. Measuring emission at wavelengths between 675-710 nm gave an exaggeration of the short-wavelength forms and measuring at longer wavelengths gave for the pea leaves an exaggeration of the 672 nm peak. In general, an energy transfer from short-wavelength Pchlide forms to long-wavelength Pchlide forms occurred, but such an energy transfer sometimes seemed to be limited as a result of a discrete location of the Pchlide spectral forms. The excitation spectra resembling the absorption spectrum most were measured at an emission wavelength of 740 nm. Measuring the excitation at 710 nm gave higher intensity of the spectra but the short-wavelength forms were accentuated. and M. R. Amirjani, C. Sundqvist.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

83-92

Source:

Photosynthetica | 2006 Volume:44 | Number:1

Harvested from:

CDK

Metadata only:

false