The present study examined the hypothesis that the extension of noxious effect of methamphetamine (MA) on maternal behavior and postnatal development on the pups may differ in dependence with time of application. Female rats were injected with MA (5 mg/kg) or saline during first (embryonic day (ED) 1-11) or second (ED 12-22) half of gestation. Our results demonstrated that MA exposure on ED 12-22 led to decreased birth weight and weight gained during lactation period relative to rats treated on ED 1-11. Both sexes treated prenatally with MA on ED 1-11 opened eyes earlier compared to animals treated on ED 12-22. As a matter of sensorimotor development application of MA on ED 1-11 impaired the righting reflex, while MA exposure on ED 12-22 impaired the performance of beam balance test in male rats. There were no differences in maternal behavior. Therefore, it seems that MA exposure in the first half of the gestation impaired the early sensorimotor development that is under control of the brain stem, while the MA exposure in the second half of gestation affected the beam balance performance that is dependent on the function of the cerebellum., M. Malinová-Ševčíková, I. Hrebíčková, E. Macúchová, E. Nová, M. Pometlová, R. Šlamberová., and Obsahuje bibliografii
Drug abuse of pregnant women is a growing problem. The effect of prenatal drug exposure may have devastating effect on development of the offsprings that may be long-term or even permanent. One of the most common drug abused by pregnant women is methamphetamine (MA), which is also the most frequently abused illicit drug in the Czech Republic. Our previous studies demonstrated that prenatal MA exposure alters behavior, cognition, pain and seizures in adult rats in sex-specific manner. Our most recent studies demonstrate that prenatal MA exposure makes adult rats more sensitive to acute injection of the same or related drugs than their controls. The aim of the present study was to examine the effect of prenatal MA exposure on drugseeking behavior of adult male rats tested in the Conditioned place preference (CPP). Adult male rats were divided to: prenatally MA-exposed (5 mg/kg daily for the entire prenatal period), prenatally saline-exposed (1 ml/kg of physiological saline) and controls (without maternal injections). The following drugs were used in the CPP test in adulthood: MA (5 mg/kg), amphetamine (5 mg/kg), cocaine (5 and 10 mg/kg), morphine (5 mg/kg), MDMA (5 mg/kg) and THC (2 mg/kg). Our data demonstrated that prenatally MA-exposed rats displayed higher amphetamine-seeking behavior than both controls. MA as well as morphine induced drug-seeking behavior of adult male rats, however this effect did not differ based on the prenatal MA exposure. In contrast, prenatal MA exposure induced rather tolerance to cocaine than sensitization after the conditioning in the CPP. MDMA and THC did not induce significant effects. Even though the present data did not fully confirmed our hypotheses, future studies are planned to test the drug-seeking behavior also in self-administration test., R. Šlamberová, ... [et al.]., and Obsahuje seznam literatury
Drug abuse during pregnancy is a growing problem in all developed countries all over the world. The drugs easily cross the placental barrier into the fetal body and are present also in the maternal milk. Therefore, it may affect the development of the child pre- as well as postnatally. The effects of prenatal drug exposure are long-lasting and persist until adulthood. The present review summarizes the clinical and experimental evidence showing how opioids and psychostimulants can affect maternal behavior of drug-abusing mother and the development of their offspring., R. Šlamberová., and Obsahuje seznam literatury
The aim of the present study was to compare the response to acute application of several drugs in adult male and female rats prenatally exposed to metham phetamine (MA). Spontaneous locomotor activity and exploratory behavior of adult male and female rats prenatally exposed to MA (5 mg/kg) or saline were tested in a Laboras apparatus (Metris B.V., Netherlands) for 1 h. Challenge dose of the examined drug [amphetamine - 5 mg/kg; cocaine - 5mg/kg; MDMA (3,4-methylenedioxymethamphetamine) - 5 mg/kg; morphine - 5 mg/kg; THC (delta9-tetrahydrocannabinol) - 2 mg/kg] or saline was injected prior to testing. Our data demonstrate that prenatal MA exposure did not affect behavior in male rats with cocaine or morphine treatment, but increased locomotion and exploration in females. Application of amphetamine and MDMA in adulthood increased activity in both sexes, while cocaine and THC only in female rats. Morphine, on the other hand, decreased the activity in the Laboras test in both sexes. As far as sex and estrous cycle is concerned, the present study shows that males were generally less active than females and also females in proestrus-estrus phase of the estrous cycle were more active than females in diestrus. In conclusion, the present study shows that the pr enatal MA exposure does not induce general sensitization but affects the sensitivity to drugs dependently to mechanism of drug action and with respect to gonadal hormones., R. Šlamberová ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Methamphetamine (MA), as a psychostimulant drug that crosses
the placental barrier, may disrupt the development of social play.
The present study aims to examine the effect of prenatal MA
(5 mg/kg) exposure during the first (gestational day (GD) 1-11)
or second (GD 12–22) halves of prenatal development of rats on
social play behavior. To investigate an acute effect of MA on
social play in adulthood, juvenile rats were exposed to a dose of
1 mg/kg MA or saline on the test day and tested for social play
for 15 min. Prenatal exposure to MA during GD 1–11 increased
social play behavior during 5-10 min interval of the test in males
but not females. Prenatal MA during GD 12–22 did not influence
social play in males nor females. However, social play occurred to
a greater extent in GD 12–22 groups compared with GD 1–11.
Acute exposure to MA eliminated playful behavior in all groups
and decreased social exploration in GD 1–11. Our results suggest
that manipulation of prenatal development during the first half of
the gestational period has a greater impact on social play
behavior than during the second half.