The study summarises the basic findings regarding non-masonry buildings in medieval Brno and the prospects for further knowledge. A modern methodological approach in the form of micromorphology in an archaeological context, particularly regarding the interiors of these buildings, was used to add more information. From more than 200 micromorphological samples taken in Brno since 2008, a total of 16 from five sites in the historical centre of the city were selected for this partial study. Temporally, this concerns the period between the early 13th and the mid-14th centuries. The basic outcome of the study is the analysis, interpretation and division of the samples into typological groups. Based on typical features, sunken parts of the structures used for a relatively long time could be divided from the above-ground structures even though in some cases the “floor sandwich” had been partly removed due to ongoing maintenance. Finally, the results were confronted with a macroscopic observation by an archaeologist and with ethnographic knowledge. It was possible to identify the more detailed microstratigraphy of the floor sandwiches than by macroscopic observations in most cases and therefore specify the interpretation of the origin of the floor sandwich.