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1. In silico, in vitro and in vivo approach in understanding the functional relationship between ergosterol and Rubisco

Creator:
Mitra, J., Narad, P., and Paul, P. K.
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
metabolity, metabolites, enzyme activity, rajče, CO2, aktivity enzymů, ergosterol, phylloplane, Rubisco, tomato, chloroplast, in silico, in vivo, large subunit, maximum carboxylation rate, 2, and 581
Language:
Multiple languages
Description:
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39) is one of the key enzymes involved in assimilation of CO2 in chloroplasts. Phylloplane microfungi and their metabolites have been reported to affect the physiology of host plants, particularly, their photosynthesis. However, information is lacking on the effect of these microflora on the physiology of chloroplasts. The current study emphasized the impact of two dominant phylloplane fungi, Aspergillus niger and Fusarium oxysporum, on activity of Rubisco in tomato chloroplasts. Ergosterol, which is a component of only fungal cell membranes and is not synthesized by plants, have been demonstrated to elicit activity of Rubisco. In the present study, it was demonstrated through in silico, in vitro, and in vivo approaches. Results demonstrated that the fungal metabolites, which contained ergosterol, could double Rubisco activity. Maximum carboxylation rate of Rubisco increased also in ergosterol-treated plants. Michaelis-Menten constant of Rubisco was also slightly affected. Ergosterol was found also to influence and enhance the binding of CO2 and ribulose-1,5-bisphosphate to Rubisco. Therefore we can postulate that the physiology of the chloroplast is probably influenced by phylloplane microfungi., J. Mitra, P. Narad, P. K. Paul., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Sodium bisulfite enhances photosynthesis in rice by inducing Rubisco activase gene expression

Creator:
Chen, Y., Jin, J.-H., Jiang, Q.-S., Yu, C.-L., Chen, J., Xu, L.-G., and Jiang, D.-A.
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
fotosyntéza, rýže, photosynthesis, rice, carboxylation efficiency, grain yield, maximum carboxylation rate, photorespiration, RuBP regeneration rate, Oryza sativa, 2, and 581
Language:
Multiple languages
Description:
To investigate how bisulfite promotes photosynthesis, a pot experiment was conducted with rice (Oryza sativa L.) plants to determine Rubisco activity and content, and Rubisco activase (RCA) gene expression after spraying NaHSO3 on rice leaves. The NaHSO3 treatment promoted significantly net photosynthetic rate (PN), carboxylation efficiency, maximum carboxylation rate, ribulose-1,5-bisphosphate regeneration rate, initial Rubisco activity, and RCA protein and mRNA concentrations. Therefore, the NaHSO3 enhancement of PN could be directly attributed to induction of RCA gene expression both at the transcription and translation levels. Thus, the increased RCA regulated the initial Rubisco activity in vivo., Y. Chen, J.-H. Jin, Q.-S. Jiang, C.-L. Yu, J. Chen, L.-G Xu, D.-A. Jiang., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public