The role of adrenergic agonists on glycogenolysis in rat hepatocyte cultures and possible involvement of NO

Title:

The role of adrenergic agonists on glycogenolysis in rat hepatocyte cultures and possible involvement of NO

Creator:

Hodis, J., Nikolína Kutinová-Canová, Petr Potměšil, Ludmila Kameníková, Eva Kmoníčková, Zdeněk Zídek, and Hassan Farghali

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:d8d82e3c-1bed-40fd-89a5-6fe6cc880775
uuid:d8d82e3c-1bed-40fd-89a5-6fe6cc880775
issn:0862-8408

Subject:

Experimentální medicína, farmakologie, oxid dusnatý, adrenalin, pharmacology, nitric oxide, adrenaline, glykogen, glykogenolýza, epinefrin, glycogen, glycogenolysis, epinephrine, iNOS, cAMP, 14, and 616-092

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Certain liver metabolic diseases point to the presence of disturbances in glycogen deposition. Epinephrine raises the cAMP level that activates protein kinase A leading to the activation of phosphorylase and glycogen breakdown. In the present report, we sought to investigate whether NO is produced during adrenoceptor agonist-induced glycogenolysis in rat hepatocytes in cultures. Isolated glycogen rich rat hepatocytes in cultures were used. NO production (NO2-) was assessed under the effect of adrenergic agonists and adrenergic agonist/antagonist pairs, dibutyryl cyclic AMP sodium-potassium salt (db-cAMP), NO synthase (NOS) inhibitors Nω-nitro-L-arginine methyl ester (L-NAME), aminoguanidine (AG) and the NO donor S-nitroso-N-acetyl penicillamine (SNAP) . The inducible NO synthase (iNOS) mRNA was examined by the reverse transcription-polymerase chain reaction (RT-PCR). Glycogenolysis was quantified by glucose levels released into medium. The amount of glucose and NO2- released by hepatocytes was increased as a result of epinephrine, phenylephrine or db-cAMP treatments. The increase in glucose and NO2- released by epinephrine or phenylephrine was blocked or reduced by prazosin pretreatment and by NOS inhibitors aminoguanidine and L-NAME. iNOS gene expression was up-regulated by epinephrine. It can be concluded that glycogenolysis occurs through α adrenoceptor stimulation and a signaling cascade may involve NO production., J. Hodis, N. Kutinová-Canová, P. Potměšil, L. Kameníková, E. Kmoníčková, Z. Zídek, H. Farghali., and Obsahuje biblografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:4

Harvested from:

CDK

Metadata only:

false