In this work, we evaluated the effect of adaptation to heat on the fall of blood pressure (BP) induced by heat shock (HS) and the interrelation between nitric oxide (NO) and heat shock protein, HSP70. Experiments were carried out on Wistar rats. It was shown that HS resulted in a generalized and transient increase in NO production (the electron paramagnetic resonance method) and a fall of BP from 113± 3 to 88± 1 mm Hg (?<0.05). Adaptation to heat itself did not affect BP, but completely prevented the NO overproduction and hypotension induced by HS. The adaptation simultaneously increased the brain NO-synthase content and induced HSP70 synthesis (the Western blot analysis) in various organs. Both the antihypotensive effects of adaptation and HSP70 accumulation were completely prevented by L-NNA, an inhibitor of NO synthesis, or quercetin, an inhibitor of HSP70 synthesis. The data suggest that adaptation to heat stimulates NO synthesis and NO activates synthesis of HSP70. HSP70, which hampers NO overproduction, thus restricts the BP fall induced by heat shock., I. Yu. Malyshev, L.A. Bayda, A.I. Trifonov, N.P. Larionov, L.D. Kubrina, V.D. Mikoyan, A.F. Vanin, E.B. Manukhina., and Obsahuje bibliografii
Chronic wound is a serious medical issue due to its high prevalence and complications; hyperbaric oxygen therapy (HBOT) is also considered in comprehensive treatment. Clinical trials, including large meta-analyses bring inconsistent results about HBOT efficacy. This review is summarizing the possible effect of HBOT on the healing of chronic wound models at the cellular level. HBOT undoubtedly escalates the production of reactive oxygen and nitrogen radicals (ROS and RNS), which underlie both the therapeutic and toxic effects of HBOT on certain tissues. HBOT paradoxically elevates the concentration of Hypoxia inducible factor (HIF) 1 by diverting the HIF-1 degradation to pathways that are independent of the oxygen concentration. Elevated HIF-1 stimulates the production of different growth factors, boosting the healing process. HBOT supports synthesis of Heat shock proteins (HSP), which are serving as chaperones of HIF-1. HBOT has antimicrobial effect, increases the effectiveness of some antibiotics, stimulates fibroblasts growth, collagen synthesis and suppresses the activity of proteolytic enzymes like matrix metalloproteinases. All effects of HBOT were investigated on cell cultures and animal models, the limitation of their translation is discussed at the end of this review
Our aim was to investigate whether hyperthermia before exercise protects against exercise-induced skeletal muscle damage. Two hyperthermia protocols were evaluated. In the first, male ICR mice were exposed to 30 min of whole-body heat in an environmental chamber at an ambient temperature of 42 °C. Heat-exposed and non-heat-exposed mice subsequently completed 60 min of downhill running on a treadmill, 24 h after exposure. Heat exposure significantly increased HSP70 and HSP25 content in the soleus muscle compared to controls. Plasma creatine kinase, muscle β-glucuronidase, and histochemical (hematoxylin and eosin stain) analysis demonstrated that muscle damage was lower in the heatexposed mice than in the non-heat-exposed mice. In the second, the effect of regional heating of the legs, by microwave diathermy, on the prevention of exercise-induced muscle damage was evaluated in male Wistar rats. Microwave-treated and nonmicrowave-treated rats again completed the running protocol 24 h after exposure. Microwave diathermy increased the muscle temperature to 40 °C, significantly increased HSP70 and HSP25 content in the soleus muscle, and significantly attenuated exercise-induced muscle damage. Therefore, hyperthermia before exercise increases skeletal muscle HSPs and attenuates the risk of exercise-induced muscle injury.
We investigated the effects of repeated hyperthermic bouts on the heat shock response of heat s hock protein ( HSP ) 72 in skeletal muscle. Rats were assigned to control and hyperthermia groups which were exposed to heated water at 42 °C. The hyperthermia group was further divided into sub -groups: a single bout (H30) or four bouts of hyperthermia for 30 min (H30x4). There was an increase in HSP72 protein content of the H30 groups in both extensor digitorum longus (EDL) and soleus muscles. Moreover, HSP72 protein expression in H30x4 group was significantly higher than in H30 group in both EDL and soleus muscles. The HSP72 mRNA was markedly increased from control levels in the H30 and H30x4 group in both types of muscles . However, HSP72 mRNA of the H30x4 group was lower than that of the H30 group in soleus muscles. Heat shock response of HSP72 is activate d even after repeated bouts of hyperthermia, with a differential regulation between muscle types., J. Lee, K. Himori, D. Tatebayashi, M. Abe, T. Yamada., and Obsahuje bibliografii
The aim of this study was to evaluate the effects of exposure to
30 daily whole body cryostimulation (WBC) on lipid metabolic
parameters and serum HSP-70 concentration. The study involved
45 volunteers, homogeneous in terms of diet and daily physical
activity. Blood samples were collected before and after the 10th,
the 20th, and the 30th session and one month after the
intervention. Total cholesterol, HDL, TG concentrations and
Apolipoprotein A-I, ApoB and HSP-70 protein levels were
determined in serum. Additionally, the LI (Lipid Index) and the
LDL level were calculated. During exposure, positive changes in
the lipid profile that included a decrease in the TCh, initiated
after the 20th WBC session with a simultaneous decrease in
TG and LDL levels, and an increase in the HDL concentration
were observed. These changes were accompanied by
a downward trend in the ApoB concentration and a decrease in
the ApoB:ApoA-I ratio after 30 sessions. The nature of these
changes persisted for a month after the exposure. The obtained
results indicate metabolic benefits that result from prolonged
exposure to cryogenic temperatures, confirming the postulate of
using WBC in the regulation of lipid metabolism and the
prevention of cardiovascular diseases.