Uridine is postulated to participate in the development of insulin resistance. Since exercise is an effective tool in the treatment of insulin resistance it appeared justified to assess the impact of maximal exercise on plasma uridine and insulin sensitivity indices (e.g. insulin and HOMA-IR) in healthy subjects. The study included forty-four healthy males (18.5±2.92 years, VO2 max 50.2±6.26 ml kg-1 min-1). Subjects performed a single maximal exercise on a bicycle ergometer. Blood samples were taken three times: immediately before exercise, immediately after exercise and at the 30th min of rest. Uridine concentrations were determined in the whole blood using high-performance liquid chromatography. Serum insulin levels were measured by a specific ELISA method. Insulin sensitivity was assessed by homeostasis model assessment method (HOMA-IR). A maximal exercise-induced increase in the concentration of uridine correlated with post-exercise increases in insulin levels and HOMA-IR. Our results indicate a relationship between the concentration of uridine in the blood and indicators of insulin sensitivity in healthy subjects. We are the first to demonstrate that a maximal exercise-induced increase in the concentration of uridine is correlated with post-exercise increases in insulin levels and HOMA-IR in healthy subjects. It appears that uridine may be an indicator of insulin resistance., W. Dudzinska, ... [et al.]., and Obsahuje seznam literatury
The aim of this study was to evaluate the effects of exposure to
30 daily whole body cryostimulation (WBC) on lipid metabolic
parameters and serum HSP-70 concentration. The study involved
45 volunteers, homogeneous in terms of diet and daily physical
activity. Blood samples were collected before and after the 10th,
the 20th, and the 30th session and one month after the
intervention. Total cholesterol, HDL, TG concentrations and
Apolipoprotein A-I, ApoB and HSP-70 protein levels were
determined in serum. Additionally, the LI (Lipid Index) and the
LDL level were calculated. During exposure, positive changes in
the lipid profile that included a decrease in the TCh, initiated
after the 20th WBC session with a simultaneous decrease in
TG and LDL levels, and an increase in the HDL concentration
were observed. These changes were accompanied by
a downward trend in the ApoB concentration and a decrease in
the ApoB:ApoA-I ratio after 30 sessions. The nature of these
changes persisted for a month after the exposure. The obtained
results indicate metabolic benefits that result from prolonged
exposure to cryogenic temperatures, confirming the postulate of
using WBC in the regulation of lipid metabolism and the
prevention of cardiovascular diseases.
Studies have shown that uridine concentration in plasma may be an indicator of uric acid production in patients with gout. It has been also postulated that uridine takes part in blood pressure regulation. Since physical exercise is an effective tool in treatment and prevention of cardio-vascular diseases that are often accompanied by hyperuricemia and hypertension, it seemed advisable to attempt to evaluate the relationship between oxypurine concentrations (Hyp, Xan and UA) and that of Urd and BP after physical exercise in healthy subjects. Sixty healthy men (17.2±1.71 years, BMI 23.2±2.31 kg m-2, VO2max 54.7±6.48 ml kg-1 min-1) took part in the study. The subjects performed a single maximal physical exercise on a bicycle ergometer. Blood for analyses was sampled three times: immediately before exercise, immediately after exercise, and in the 30th min of rest. Concentrations of uridine and hypoxanthine, xanthine and uric acid were determined in whole blood using high-performance liquid chromatography. We have shown in this study that the maximal exercise-induced increase of uridine concentration correlates with the post-exercise increase of uric acid concentration and systolic blood pressure. The results of our study show a relationship between uridine concentration in blood and uric acid concentration and blood pressure. We have been the first to demonstrate that a maximal exercise-induced increase in uridine concentration is correlated with the post-exercise and recovery-continued increase of uric acid concentration in healthy subjects. Thus, it appears that uridine may be an indicator of post-exercise hyperuricemia and blood pressure., W. Dudzinska, A. Lubkowska, B. Dolegowska, M. Suska, M. Janiak., and Obsahuje bibliografii