Female genitalia of lygaeid bugs are characterized by a tube-shaped ductus receptaculi (spermathecal duct) connecting the bursa copulatrix with the highly coiled receptaculum seminis (spermatheca). In this study the morphology and functional anatomy of these structures in Lygaeus simulans were examined by light-, fluorescence- and electron microscopy. In addition, copulating animals were freeze fixed and their interconnected genital structures observed using light microscopy. The ductus receptaculi is separated from the receptaculum seminis by a complicated valve. The valve is nearly surrounded by the spermathecal muscle, which controls its opening. The ductus receptaculi leads into the proximal convoluted tube of the receptaculum seminis. Both the ductus receptaculi and the convoluted tube are composed of a single layer of epithelial cells lined by a thick electron dense apical cuticle. The distal part of the receptaculum seminis is a brownish, irregularly coiled, blind ending canal made of small epithelial cells covered with cuticle. Big glands are present in the epidermal layer. The cuticle of this distal part is much thinner and features concentric lamellae. The lumen of the receptaculum seminis cannot be expanded. For transfering sperm into the receptaculum the male aedeagus is equipped with a long, tube-like, sclerotized appendix (processus gonopori), which enlarges the ductus receptaculi considerably during copulation. For successful insemination the tip of the processus gonopori has to pass the valve. The convoluted tube, the valve and the surrounding spermathecal muscle may enable females to control insemination and egg fertilization.
In the laboratory, not all females of the seed bug Lygaeus simulans Deckert, 1985, produced fertilized eggs after copulation: 26.7% of the females were not inseminated and 5% were inseminated but did not lay fertilized eggs; only in 40% of the couples did copulation result in fertile eggs. The remaining 28.3% of couples refrained from mating. Duration of copulation was associated with insemination and fertilization: (i) fertile eggs were produced by only one couple that copulated for less than 60 min and all those that copulated for more than 360 min, (ii) probability of fertilization increased steadily with duration of copulation between 60 and 360 min, and (iii) duration of copulation was significantly different for couples that showed different insemination status.
A possible morphological explanation for this rime dependency was revealed by examining the genitalia of 69 couples freeze-fixed in copula after different periods in copulation. Because of the intricate structure of the genitalia in L. simulans, a male takes a long time to manoeuver its intromittent organ into the narrow insemination duct of the female. Only if completely inserted is the tip of the intromittent organ close enough for successful ejaculation of sperm into the spermatheca. The freeze-fixing experiment revealed that it usually took the male more than 30 min to locate the entrance to the insemination duct and another 30 min for full penetration. This explains why copulations that lasted less than 60 min failed, since insemination began only after intromission was complete.
The experiments, therefore, indicated that there is a relationship between the complex morphology of the genitalia and the low rates of insemination and fertilization in L. simulans., Thomas Micholitsch, Peter Krügel, Günther Pass, 7 obrázků, 1 tab., and Lit.
Insect genital characters are extensively used in species level taxonomy, and their value in species delimitation is great. Based on the lock-and-key hypothesis and that genital differences function as a mechanical isolation system between species, the value of genital characters has been thought to be superior to non-genital characters. Although geographical and other kind of intraspecific variation of genitalia is often assumed very moderate, its real extent is insufficiently investigated. We examined patterns of morphological variation in the male genitalia of the tortricid moth Pammene luedersiana, using geometric morphometric tools including thin-plate spline deformation grids, and found significant variation. This variation is continuous both within and between populations. No systematic shape variation was observed between populations, but genital size showed some geographic variability. The results suggest that genital morphology is not constant and should therefore be used with caution in lepidopteran taxonomy.
We present a molecular phylogeny incorporating all five species of the Palaearctic geometrid genus Lythria, based on a 2810-bp combined data matrix comprising the full sequence of the mitochondrial gene cytochrome oxidase subunit one (COI) and fragments of the nuclear genes elongation factor 1 alpha (EF-1α) and wingless (wgl). L. venustata, which was recently rediscovered from Kazakhstan, is shown to be sister taxon to all other members of the genus. The remaining species within the genus form two pairs of sister species: L. purpuraria groups together with L. plumularia, and L. cruentaria with L. sanguinaria. The phylogeny is well supported by characters of the male genitalia of all Lythria species. In addition to the molecular phylogeny of the genus Lythria, we illustrate the external appearance of L. venustata for the first time and describe the anatomy of its male genitalia.