This work aimed to study the effects of commercial doses of the fungicide, Mancozeb, on the photosynthetic apparatus of lettuce young leaves (YL) and expanded leaves (EL). Seven days after Mancozeb application, chlorophyll a fluorescence, pigment contents, lipid peroxidation, and proline content were evaluated. Independently of leaf age, Mancozeb treatment reduced the efficiency of photosystem II photochemistry, increased the nonphotochemical quenching and proline content, decreased pigment contents, and induced lipid peroxidation. Moreover, EL showed a more stable photosynthetic apparatus, less prone to oxidative damages compared with YL. The parameters measured proved to be good markers for the rapid and preliminary diagnosis of fungicide toxicity., M. C. Dias, P. Figueiredo, I. F. Duarte, A. M. Gil, C. Santos., and Obsahuje bibliografii