The article deals with content and provenance interpretation of the oldest parchment manuscript fragment which is deposited in the Library of the Department of Auxiliary Historical Sciences and Archive Studies at the Faculty of Arts, MU. The piece is identified as a fragment of Lectionaria officii, which was probably written by the Order of Saint Benedict Benedictines in the first half of the 12th century, which was taken from the binding of one of the books from Bohemian or Moravian Franciscan library.