Participation of P-dependent and P-independent glutaminases in rat kidney ammoniagenesis and their modulation by metabolic acidosis, hippurate and insulin

Title:

Participation of P-dependent and P-independent glutaminases in rat kidney ammoniagenesis and their modulation by metabolic acidosis, hippurate and insulin

Creator:

Krivošíková, Z., Spustová, V., and Dzúrik, R.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:2993684e-eb45-423b-86df-26b486588551
uuid:2993684e-eb45-423b-86df-26b486588551

Subject:

ammoniagenesis, P-independent glutaminase, P-dependent glutaminase, metabolic acidosis, hippurate, and insulin

Type:

article, model:article, and TEXT

Description:

The key regulatory enzymes of kidney ammoniagenesis appear to be P-dependent (PDG) and P-independent (PIG) glutaminases. While the participation of PDG has been satisfactorily elucidated, the significance of PIG remains doubtful. Rat kidney cortex slices synthesized ammonia even under basal conditions. Metabolic acidosis, hippurate and insulin stimulated ammonia production. Under basal conditions, PDG activity in kidney homogenate, was twice as high as PIG activity. Metabolic acidosis stimulated ammonia production by the stimulation of both PDG (100 %) and PIG (57 %) activities. Hippurate stimulated only PIG activity both under basal conditions (90 %) and in metabolic acidosis (52 %), while it inhibited PDG activity only insignificantly under basal conditions and markedly (53 %) in metabolic acidosis. Insulin stimulated both PIG and PDG activities under basal conditions as well as in metabolic acidosis and potentiated the PIG stimulation by hippurate while it potentiated the hippurate inhibition of PDG both under basal conditions and in acidotic rats. In conclusion, both PDG and PIG participate in ammoniagenesis and are stimulated by metabolic acidosis and insulin. Hippurate stimulates PIG, while it inhibits PDG in metabolic acidosis and even after insulin administration. The effect of hippurate appears to be of physiological interest.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1998 Volume:47 | Number:3

Harvested from:

CDK

Metadata only:

false