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4. Case 1-2012: ANCA associated glomerulonephritis in combination with IgG4 Positive mediastinal mass in a patient with ankylosing spondylitis treated with TNF alpha inhibitors

Creator:
Tošovský, Marian, Bradna, Petr, Laco, Jan, Podhola, Miroslav, Soukup, Tomáš, Brožík, Jan, and Šteiner, Ivo
Format:
braille, electronic resource, remote, and elektronický zdroj
Type:
model:article, article, Text, statistics, kongresy jako téma, práce podpořená grantem, and TEXT
Subject:
protilátky proti cytoplazmě neutrofilů--krev, monoklonální protilátky humanizované--terapeutické užití, fokálně segmentální glomeruloskleróza--diagnóza--imunologie--komplikace, lidé, imunoglobulin G--krev, mužské pohlaví, nemoci mediastina--diagnóza--imunologie--komplikace, lidé středního věku, ankylózující spondylitida--farmakoterapie--komplikace, and TNF-alfa--antagonisté a inhibitory
Language:
English
Description:
Professor Ivo Šteiner, MD, PhD - editor, Marian Tošovský, Petr Bradna, Jan Laco, Miroslav Podhola, Tomáš Soukup, Jan Brožík and Literatura 15
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

6. Human plasma and human platelet-rich plasma as a substitute for fetal calf serum during long-term cultivation of mesenchymal dental pulp stem cells

Creator:
Suchánková Kleplová, Tereza, Soukup, Tomáš, Řeháček, Vít, and Suchánek, Jakub
Format:
print, text, and regular print
Type:
model:article, article, Text, časopisecké články, práce podpořená grantem, and TEXT
Subject:
zvířata, skot, buněčné kultury--metody, proliferace buněk, buňky kultivované, kultivační média, zubní dřeň--cytologie, fetální krev, lidé, mezenchymální stromální buňky, plazma bohatá na destičky, and čas
Language:
English
Description:
AIMS: Our aims were to isolate and cultivate mesenchymal dental pulp stem cells (DPSC) in various media enriched with human blood components, and subsequently to investigate their basic biological properties. METHODS: DPSC were cultivated in five different media based on α MEM containing different concentrations of human plasma (HP), platelet-rich plasma (PRP), or fetal calf serum (FCS). The DPSC biological properties were examined periodically. RESULTS: We cultivated DPSC in the various cultivation media over 15 population doublings except for the medium supplemented with 10% HP. Our results showed that DPSC cultivated in medium supplemented with 10% PRP showed the shortest average population doubling time (DT) (28.6 ± 4.6 hours), in contrast to DPSC cultivated in 10% HP which indicated the longest DT (156.2 ± 17.8 hours); hence this part of the experiment had been cancelled in the 6th passage. DPSC cultivated in media with 2% FCS+ITS (DT 47.3 ± 10.4 hours), 2% PRP (DT 40.1 ± 5.7 hours) and 2% HP (DT 49.0 ± 15.2 hours) showed almost the same proliferative activity. DPSC's viability in the 9th passage was over 90% except for the DPSC cultivated in the 10% HP media. CONCLUSIONS: We proved that human blood components are suitable substitution for FCS in cultivation media for long-term DPSC cultivation. and T. Suchánková Kleplová, T. Soukup, V. Řeháček, J. Suchánek
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

8. The effect of fetal calf serum on human dental pulp stem cells

Creator:
Suchánek, Jakub, Suchánková Kleplová, Tereza, Kapitán, Martin, and Soukup, Tomáš
Format:
print, text, and regular print
Type:
model:article, article, Text, srovnávací studie, časopisecké články, práce podpořená grantem, and TEXT
Subject:
zvířata, skot, proliferace buněk, buňky kultivované, zubní dřeň--cytologie, ženské pohlaví, fetální krev--cytologie, lidé, mužské pohlaví, kmenové buňky--cytologie, and mladý dospělý
Language:
English
Description:
AIMS: Authors studied potential side effects of fetal calf serum (FCS) in cultivation media on human dental pulp stem cells (DPSC) during long-term cultivation. METHODS: Two lines of DPSC obtained healthy donors (male 22 years, female 23 years) were used. Both lines were cultivated under standard cultivation conditions in four different media containing 10% or 2% FCS and substituted with growth factors. During long-term cultivation proliferation ability, karyotype and phenotype of DPSC were measured. RESULTS: Both lines of DPSC cultivated in a media containing 2% FCS and ITS supplement showed the highest number of population doublings. On the other hand the proliferation rate of DPSC cultivated in a media with 2% FCS without ITS supplement was slowest. Proliferation rate of DPSC cultivated in 10% FCS media with or without FGF-2 was comparable. DPSC cultivated in a media with 10% FCS showed a significantly higher amount of chromosomal aberrations. These chromosomal aberrations do not seem to be clonal but surprisingly we found large amounts of tetraploid cells in the 9th passage in both media containing 10% FCS. CONCLUSIONS: Our study proved that cultivation of DPSC in media containing higher concentration of FCS has critical side effects on cell chromosomal stability. and J. Suchánek, TS. Kleplová, M. Kapitán, T. Soukup
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

9. The VEGF and BMP-2 Levels in Patients with Ankylosing Spondylitis and the Relationship to Treatment with Tumour Necrosis Factor Alpha Inhibitors

Creator:
Tošovský, Marian, Bradna, Petr, Andrýs, Ctirad, Andrýsová, Kateřina, Čermáková, Eva, and Soukup, Tomáš
Format:
print, text, and regular print
Type:
model:article, article, Text, časopisecké články, and TEXT
Subject:
dospělí, kostní morfogenetický protein 2--krev, C-reaktivní protein--metabolismus, progrese nemoci, ELISA, ženské pohlaví, lidé, mužské pohlaví, lidé středního věku, ankylózující spondylitida--krev--farmakoterapie, výsledek terapie, TNF-alfa--antagonisté a inhibitory, and vaskulární endoteliální růstový faktor A--krev
Language:
English
Description:
Introduction: Ankylosing spondylitis (AS) is an inflammatory rheumatic disease characterized by the development of osteoproductive changes in the spine which could possibly result in ankylosis. Treatment with tumour necrosis factor alpha (TNFα) inhibitors has proved to be an important step forward in the treatment of this disease, but for the time being it is not clear whether it favourably influences radiographic progression of the disease. Vascular endothelial growth factor most probably plays a role in the development of osteoproductive changes and recently its predictive influence on radiographic progression has been demonstrated. Bone morphogenic protein 2 (BMP-2) participates in the regulation of bone proliferation and its increased serum level has been demonstrated in patients with advanced AS and correlated with the degree of radiographic changes. Aim: The study aims to evaluate the VEGF and BMP-2 levels in patients with ankylosing spondylitis and how these levels relate to the concurrent treatment with TNFα inhibitors. Methods: Sera were evaluated from patients at the Rheumatologic Clinic of the Hradec Králové Faculty Hospital who fulfilled the modified New York Criteria for AS (n = 55). In these patients, the parameters of the activity of the disease (BASDAI = Bath Ankylosing Spondylitis Disease Activity Index, CRP = C-reactive protein) and the concurrent therapy (TNFα inhibitors, n = 21, vs. non-anti TNFα, n = 34) were recorded. The levels of VEGF and BMP-2 were analyzed using the ELISA method. Results: In patients treated with TNFα inhibitors, a significantly lower VEGF level was found when compared to untreated patients (140.3 (109.4; 262.2) vs. 261 (172.4; 396.6) pg/ml; p = 0.02). No difference was found between BMP-2 levels in both groups (treated vs. untreated patients) (254.8 (2301; 267.3) vs. 261.1 (248.6; 273.5) pg/ml; p = 0.24). A correlation analysis did not reveal any relationship between VEG F and BMP-2 (r = 0.057; p = 0.68). Serum levels of VEGF correlated with serum levels of CRP (r = 0.56; p = 0.00001) and the BASDAI value (r = 0.33; p = 0.015). Conclusion: Significantly lower VEGF levels were found in patients treated with TNFα inhibitors versus the untreated patients. These findings are in harmony with some hitherto published analyses and may give evidence of a favourable effect of TNFα inhibitors on radiographic progression. Neither influence on the BMP-2 level by treatment with TNFα inhibitors nor correlation with VEGF levels was demonstrated. and M. Tošovský, P. Bradna, C. Andrýs, K. Andrýsová, E. Čermáková, T. Soukup
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public