The effect of fetal calf serum on human dental pulp stem cells

Title:

The effect of fetal calf serum on human dental pulp stem cells
The effect of fetal calf serum on human dental pulp stem cells

Creator:

Suchánek, Jakub, Suchánková Kleplová, Tereza, Kapitán, Martin, and Soukup, Tomáš

Contributor:

Suchánek, Jakub , 1981-, Suchánková Kleplová, Tereza, Kapitán, Martin , 1984-, and Soukup, Tomáš , 1980-

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bmc14059445-ae9183f4-3467-476f-afb7-c128116e786d
uuid:bmc14059445-ae9183f4-3467-476f-afb7-c128116e786d
local:bmc14059445
https://www.lfhk.cuni.cz/Faculty/Publications/Acta-Medica/Archive/2013/
local: bmc14059445

Subject:

zvířata, skot, proliferace buněk, buňky kultivované, zubní dřeň--cytologie, ženské pohlaví, fetální krev--cytologie, lidé, mužské pohlaví, kmenové buňky--cytologie, and mladý dospělý

Type:

model:article, article, Text, srovnávací studie, časopisecké články, práce podpořená grantem, and TEXT

Format:

print, text, and regular print

Description:

AIMS: Authors studied potential side effects of fetal calf serum (FCS) in cultivation media on human dental pulp stem cells (DPSC) during long-term cultivation. METHODS: Two lines of DPSC obtained healthy donors (male 22 years, female 23 years) were used. Both lines were cultivated under standard cultivation conditions in four different media containing 10% or 2% FCS and substituted with growth factors. During long-term cultivation proliferation ability, karyotype and phenotype of DPSC were measured. RESULTS: Both lines of DPSC cultivated in a media containing 2% FCS and ITS supplement showed the highest number of population doublings. On the other hand the proliferation rate of DPSC cultivated in a media with 2% FCS without ITS supplement was slowest. Proliferation rate of DPSC cultivated in 10% FCS media with or without FGF-2 was comparable. DPSC cultivated in a media with 10% FCS showed a significantly higher amount of chromosomal aberrations. These chromosomal aberrations do not seem to be clonal but surprisingly we found large amounts of tetraploid cells in the 9th passage in both media containing 10% FCS. CONCLUSIONS: Our study proved that cultivation of DPSC in media containing higher concentration of FCS has critical side effects on cell chromosomal stability. and J. Suchánek, TS. Kleplová, M. Kapitán, T. Soukup

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Relation:

Acta medica (Hradec Králové) / Universitas Carolina, Facultas Medica Hradec Králové--MED00010947

Source:

Acta medica (Hradec Králové) | 2013 Volume:56 | Number:4

Harvested from:

CDK

Metadata only:

false

Date:

2013