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Start Over Creator Song, Yue

1. Distribution and diversity of Wolbachia in different populations of the wheat aphid Sitobion miscanthi (Hemiptera: Aphididae) in China

Creator:
Wang, Zhe, Shen, Zuo-Rui, Song, Yue, Liu, Hong-Yue, and Li, Zheng-Xi
Type:
article, model:article, and TEXT
Subject:
Cytoplasmic symbiont, wheat aphid, Wolbachia, Sitobion miscanthi, distribution, diversity, China, and 16s rDNA gene
Language:
English
Description:
Wolbachia is a widely distributed intracellular symbiont in the reproductive tissues of arthropods. The wheat aphid Sitobion miscanthi (Takahashi) is an important agricultural pest worldwide. Wolbachia was detected in different populations of S. miscanthi in China using 16s rDNA and wsp-specific primers. Of eighteen populations eleven were infected with Wolbachia. Several strains of Wolbachia infected these S. miscanthi populations. Of the eleven infected populations, four were infected with only one Wolbachia strain and seven with double infections. This is the most systematic survey of the distribution of Wolbachia in the wheat aphid.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

2. Molecular differentiation of the B biotype from other biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae), based on internally transcribed spacer 1 sequences

Creator:
Li, Zhengxi, Hu, Dunxiao, Song, Yue, and Shen, Zuorui
Type:
article, model:article, and TEXT
Subject:
Bemisia tabaci, B Biotype, ITS1, and molecular identification
Language:
English
Description:
Bemisia tabaci (Gennadius) is a worldwide pest of vegetable, ornamental and field crops. Biotype B of B. tabaci, which is economically most important of the biotypes, is distinct from all other biotypes (non-B biotypes). Fourteen populations of B. tabaci were collected from different localities and host plants in the Chinese mainland and Taiwan, namely TWYDH (tassel flower, Taiwan), HNYC (tobacco, Hainan), GXNG (pumpkin, Guangxi), GDYPH (poinsettia, Guangdong), GDBSM (croton, Guangdong), GDFS (Chinese hibiscus, Guangdong), SHYPH (poinsettia, Shanghai), FJGS (sweet potato, Fujian), SDFQ (tomato, Shandong), BJXHL (squash, Beijing), XJQZ (eggplant, Xinjiang), XJYPH (poinsettia, Xinjiang), XJJM (abutilon, Xinjiang) and XJMH (cotton, Xinjiang). The internally transcribed spacer 1 sequences (ITS1) of ribosomal DNA of B biotype and other biotypes were sequenced and analyzed. The B biotype-specific primers were then designed for rapid identification of B biotype of B. tabaci. The results show that the diagnostic primer only gave a positive result with the B biotype. This is the first report of a rapid means of identifying B. tabaci B biotype using a diagnostic primer based on ribosomal DNA. This protocol is especially useful for identifying the B biotype in Bemisia populations consisting of several biotypes.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public