In order to investigate the effect of large isoform of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activase (RuBPCO-A) on photosynthesis, cDNA of the enzyme (rca) was transferred to rice cultivars (Oryza sativa f. japonica cv. Nipponbare) under the control of RuBPCO small subunit gene promoter (rbcS) via Agrobacterium tumefaciens-mediated transformation. Transgenic rice plants were identified by polymerase chain reaction (PCR) and Southern and Western blot analyses. Net photosynthetic rate (PN ) values of the T1 transgenic lines 34 (T34) and 40 (T40) were 45.26 and 46.32 % higher than that of the control plants, respectively. At the same time, their carboxylation efficiency and RuBPCO initial activity, quantum yield of electron transport in photosystem 2 (ΦPS2), and steady state photochemical fluorescence quenching (qP) increased. In addition, heading time of the transgenic rice was advanced. Thus increasing the amount of large isoform of RuBPCO-A in the transgenic rice might have a stimulatory effect on both photosynthesis and plant growth. and H. R. Wu ... [et al.].
Příspěvek poskytuje přehlednou informaci o základních fyzikálních principech, na nichž pracují současné hmotnostní spektrometry, a informaci o základních principech některých důležitých soudobých hmotnostně spektrálních metod v biologickém výzkumu., Jiří Knížek, Zdeněk Půlpán, Martin Hubálek, Ladislav Beránek, Petr Pokorný., and Obsahuje seznam literatury
Specimens of the elasmobranch, Isurus oxyrinchus Rafinesque, captured in 1999 in the state of Santa Catarina, Brazil, were parasitized with the poecilacanthoid trypanorhynch cestode Gymnorhynchus isuri Robinson, 1959, that is redescribed here. New details of scolex and proglottid morphology are given. These details are mainly related to tentacle armature, terminal genitalia and observations of external morphology of proglottids.