Expression of the large isoform of ribulose-1,5-bisphosphate carboxylase/oxygenase activase gene driven by rbcS promoter in Oryza sativa enhances the photosynthetic capacity

Title:

Expression of the large isoform of ribulose-1,5-bisphosphate carboxylase/oxygenase activase gene driven by rbcS promoter in Oryza sativa enhances the photosynthetic capacity

Creator:

Wu, H. R., Ding, Z. S., Li, L. B., Jing, Y. X., and Kuang, T. Y.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:b63071fa-e222-46ad-a7a2-7c5a631a40b4
uuid:b63071fa-e222-46ad-a7a2-7c5a631a40b4
issn:0300-3604
doi:10.1007/s11099-006-0008-7

Subject:

carboxylation efficiency, chlorophyll fluorescence, net photosynthetic rate, photosystem 2, and transgenic rice

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

In order to investigate the effect of large isoform of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activase (RuBPCO-A) on photosynthesis, cDNA of the enzyme (rca) was transferred to rice cultivars (Oryza sativa f. japonica cv. Nipponbare) under the control of RuBPCO small subunit gene promoter (rbcS) via Agrobacterium tumefaciens-mediated transformation. Transgenic rice plants were identified by polymerase chain reaction (PCR) and Southern and Western blot analyses. Net photosynthetic rate (PN ) values of the T1 transgenic lines 34 (T34) and 40 (T40) were 45.26 and 46.32 % higher than that of the control plants, respectively. At the same time, their carboxylation efficiency and RuBPCO initial activity, quantum yield of electron transport in photosystem 2 (ΦPS2), and steady state photochemical fluorescence quenching (qP) increased. In addition, heading time of the transgenic rice was advanced. Thus increasing the amount of large isoform of RuBPCO-A in the transgenic rice might have a stimulatory effect on both photosynthesis and plant growth. and H. R. Wu ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

208-214

Source:

Photosynthetica | 2006 Volume:44 | Number:2

Harvested from:

CDK

Metadata only:

false