A new non-uremic rat model of long-term peritoneal dialysis

Title:

A new non-uremic rat model of long-term peritoneal dialysis

Creator:

Peng, Y.-M., Shu, Z.-J., Xiao, L., Sun, L., Tang, W.-B., Huang, Y., Liu, Y.-H., Li, J., Ling, G.-H., Xu, X.-Q., Halmurat, U., and Liu, F.-Y.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:f25bca8c-07b5-4db3-8908-f1d907734bef
uuid:f25bca8c-07b5-4db3-8908-f1d907734bef

Subject:

Fyziologie člověka a srovnávací fyziologie, peritoneální dialýza, dialyzační roztoky, glukóza, peritoneal dialysis, dialyzing solutions, glucose, rat model, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Together with the development of peritoneal dialysis (PD), appropriate animal models play an important role in the investigation of physiological, pathophysiological and clinical aspects of PD. However, there is still not an ideal experimental PD animal model. In this study, 45 Sprague-Dawley rats were divided into three groups. Grou p 1 (n=15) was receiving daily peritoneal injection through the catheter connected to the abdominal cavity, using PD solution containing 3.86 % D-glucose. Group 2 (n=15) was receiving daily peritoneal injection of 0.9 % physiological saline through a catheter. Group 3 (n=15), which was subjected to sham operation, served as controls. Our results showed that WBC counts in peritoneal effluent of Group 1 were slightly higher than those of Group 2 and control group, respectively (p<0.05). However, there was no episode of infection in any group. In addition, there was no significant difference in neutrophils fractions among these three groups. Hematoxylin-eosin and Masson’s trichrome staining demonstrated a dramatic increase in thickness of the mesothelium-to-muscle layer of peritoneum exposed to high glucose (Group 1) compared to Group 2 and controls (p<0.01). These data indicated that we established a novel rat model of PD with a modified catheter insertion method. This model is more practical, easy to operate, not too expensive and it will facilitate the investigate of long-term effects of PD., Y.-M. Peng ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2011 Volume:60 | Number:1

Harvested from:

CDK

Metadata only:

false