Chromosomal differentiation among bisexual European species of Saga (Orthoptera: Tettigoniidae: Saginae) detected by both classical and molecular methods

Title:

Chromosomal differentiation among bisexual European species of Saga (Orthoptera: Tettigoniidae: Saginae) detected by both classical and molecular methods

Creator:

Warchałowska-Śliwa, Elżbieta, Grzywacz, Beata, Maryańska- Nadachowska, Anna, Karamysheva, Tatjana V., Rubtsov, Nikolai, and Chobanov , Dragan P.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:df24d141-c17c-4af9-afdf-4602e1e1c8fb
uuid:df24d141-c17c-4af9-afdf-4602e1e1c8fb

Subject:

Orthoptera, Saginae, Saga, karyotype, Ag-NOR, FISH, chromosome, rDNA, and telomeric repeats

Type:

article, model:article, and TEXT

Description:

We report the karyotype characteristics including chromosome numbers of Saga campbelli campbelli, S. c. gracilis, and S. rammei using the following classical cytogenetic methods: C-banding, silver staining, and fluorochrome staining DAPI and CMA3. We also present FISH data showing the distribution of telomeric repeats and 18S rDNA on the chromosomes of these species and the results of similar studies cited in the literature on S. hellenica, S. natoliae, and S. rhodiensis. The five European Saga species exhibit a high rate of karyotype evolution. In addition to changes in chromosome number and morphology (by chromosomal inversion and/or chromosome fusion), interspecific autosomal differentiation involved changes in the distribution and quantity of constitutive heterochromatin and GC-rich regions, as well as the number and location of NORs. In the present study we focused on testing a hypothetical model of karyotype evolution in Saga, with particular reference to the cytogenetic mapping of rDNA and telomeric sequences. Variation in the distribution of rDNA and location of Ag-NORs are novel phylogenetic markers for the genus Saga.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2009 Volume:106 | Number:1

Harvested from:

CDK

Metadata only:

false