Increased thermal stability of photosystem II and the macro-organization of thylakoid membranes, induced by co-solutes, associated with changes in the lipid-phase behaviour of thylakoid membranes

Title:

Increased thermal stability of photosystem II and the macro-organization of thylakoid membranes, induced by co-solutes, associated with changes in the lipid-phase behaviour of thylakoid membranes

Creator:

Kotakis, C., Akhtar, P., Zsiros, O., Garab, G., and Lambrev, P. H.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7ac59add-2064-4a70-bb47-30c9312f4d86
uuid:7ac59add-2064-4a70-bb47-30c9312f4d86
issn:0300-3604
doi:10.1007/s11099-018-0782-z

Subject:

fotosyntéza, photosynthesis, circular dichroism, merocyanine-540, non-bilayer lipids, osmolyte, time-resolved fluorescence spectroscopy, 2, and 581

Type:

model:article and TEXT

Format:

print, bez média, and svazek

Description:

The principal function of the thylakoid membrane depends on the integrity of the lipid bilayer, yet almost half of the thylakoid lipids are of non-bilayer-forming type, whose exact functions are not fully understood. Non-bilayer lipids can be extruded from the membrane in the presence of high concentrations of co-solutes. We applied 2 M sucrose to induce lipid phase separation in isolated thylakoid membranes, following consequent structural and physiological effects. Circular dichroism spectroscopy indicated significant changes in the chiral macro-arrangement of the pigment-protein complexes, which were reversed after washing out the co-solute. Similarly, merocyanine-540 fluorescence suggested reversible changes in the lipid phases. The PSII function, as tested by chlorophyll fluorescence induction transients and time-resolved fluorescence, was almost unaffected. However, the presence of sucrose dramatically increased the PSII thermostability, which can partly be explained by a direct osmolyte effect and partly by the lipid phase separation stabilizing the stacked membrane., C. Kotakis, P. Akhtar, O. Zsiros, G. Garab, P. H. Lambrev., and Obsahuje bibliografické odkazy

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

254-264

Source:

Photosynthetica | 2018 Volume:56 | Number:1

Harvested from:

CDK

Metadata only:

false