The seasonal diet composition of otters (Lutra lutra) living by an eutrophic fish pond system, a wetland alder forest, and a slow-flowing stream located in south-west Hungary was investigated by spraint analysis (n = 801, 116 and 234 samples respectively). Both percentage relative frequency of occurrence (O%) and biomass (B%) of food items (calculated by coefficients of digestibility) were estimated. The food composition of the otters living by the three areas differed significantly (PAstacus spp.) was dominant (54 O% and 62 B%). Close correlation was found between relative frequency of occurrence and biomass of food items.