Mechanical response of porous scaffolds for cartilage engineering

Title:

Mechanical response of porous scaffolds for cartilage engineering

Creator:

Josef Jančář, Slovíková, A., Evžen Amler, Petr Krupa, Helga Kecová, Ladislav Plánka, Petr Gál, and Alois Nečas

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:4fca7784-f532-4a50-8a62-899d1d14cb9b
uuid:4fca7784-f532-4a50-8a62-899d1d14cb9b
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, nanovlákna, kyselina hyaluronová, mezenchymální kmenové buňky, nanofibers, hyaluronic acid, mesenchymal stem cells, chitosan, hydroxyapatite, joint resurfacing, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

a1_Mechanical properties of scaffolds seeded with mesenchymal stem cells used for cartilage repair seem to be one of the critical factors in possible joint resurfacing. In this paper, the effect of adding hyaluronic acid, hydroxyapatite nanoparticles or chitosan nanofibers into the cross-linked collagen I on the mechanical response of the lyophilized porous scaffold has been investigated in the dry state at 37 oC under tensile loading. Statistical significance of the results was evaluated using ANOVA analysis. The results showed that the addition of hyaluronic acid significantly (p<<0.05) reduced the tensile elastic modulus and enhanced the strength and deformation to failure of the modified cross-linked collagen I under the used test conditions. On the other hand, addition of hydroxyapatite nanoparticles and chitosan nanofibers, respectively, increased the elastic modulus of the modified collagen ten-fold and four-fold, respectively. Hydroxyapatite caused significant reduction in the ultimate deformation at break while chitosan nanofibers enhanced the ultimate deformation under tensile loading substantially (p<<0.05). The ultimate tensile deformation was significantly (p<<0.05) increased by addition of the chitosan nanofibers. The enhanced elastic modulus of the scaffold was translated into enhanced resistance of the porous scaffolds against mechanical load compared to scaffolds based on cross-linked neat collagen or collagen with hyaluronic acid with similar porosity. It can be concluded that enhancing the rigidity of the compact scaffold material by adding rigid chitosan nanofibers can improve the resistance of the porous scaffolds against compressive loading, which can provide more structural protection to the seeded mesenchymal stem cells when the construct is implanted into a lesion., a2_Moreover, scaffolds with chitosan nanofibers seemed to enhance cell growth compared to the neat collagen I when tested in vitro as well as the scaffold stability, extending its resorption to more than 10 weeks., J. Jančář, A. Slovíková, E. Amler, P. Krupa, H. Kecová, L. Plánka, P. Gál, A. Nečas., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:Suppl 1

Harvested from:

CDK

Metadata only:

false