Effect of somatostatin on repair of ionizing radiation-induced DNA damage in pituitary adenoma cells GH3

Title:

Effect of somatostatin on repair of ionizing radiation-induced DNA damage in pituitary adenoma cells GH3

Creator:

Martina Řezáčová, Jan Čáp, Doris Vokurková, Emilie Lukášová, Jiřina Vávrová, Jaroslav Cerman, Vladimír Mašín, and Mazánková, N.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:1f4f78e1-e6fe-4710-b889-1b6e995e427a
uuid:1f4f78e1-e6fe-4710-b889-1b6e995e427a
issn:0862-8408

Subject:

Biochemie. Molekulární biologie. Biofyzika, biofyzika, ionizující záření, radioterapie, apoptóza, biophysics, ionizing radiation, radiotherapy, apoptosis, agromegalie, somatostatin, radiosurgery, H2A.X, Nbs1, GH3 cells, 2, and 577

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Ionizing radiation and somatostatin analogues are used for acromegaly treatment to achieve normalization or reduction of growth hormone hypersecretion and tumor shrinkage. In this study, we investigated a combination of somatostatin (SS14) with ionizing radiation of 60Co and its effect on reparation of radiation-induced damage and cell death of somatomammotroph pituitary cells GH3. Doses of γ-radiation 20-50 Gy were shown to inhibit proliferation and induce apoptosis in GH3 cells regardless of somatostatin presence. It has been found that the D0 value for GH3 cells was 2.5 Gy. Somatostatin treatment increased radiosensitivity of GH3 cells, so that D0 value decreased to 2.2 Gy. We detected quick phosphorylation of histone H2A.X upon irradiation by the dose 20 Gy and its colocalization with phosphorylated protein Nbs-1 in the site of double strand break of DNA (DSB). Number of DSB decreased significantly 24 h after irradiation, however, clearly distinguished foci persisted, indicating non repaired DSB, after irradiation alone or after combined treatment by irradiation and SS14. We found that SS14 alone triggers phosphorylation of Nbs1 (p-Nbs1), which correlates with antiproliferative effect of SS14. Irradiation also increased the presence of p-Nbs1. Most intensive phosphorylation of Nbs1 was detected after combined treatment of irradiation and SS14. The decrease of the number of the DSB foci 24 h after treatment shows a significant capacity of repair systems of GH3 cells. In spite of this, large number of unrepaired DSB persists for 24 h after the treatment. We conclude that SS14 does not have a radioprotective effect on somatomammotroph GH3 cells., M. Řezáčová, J. Čáp, D. Vokurková, E. Lukášová, J. Vávrová, J. Cerman, V. Mašín, N. Mazánková., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:2

Harvested from:

CDK

Metadata only:

false